Rheims H, Spröer C, Rainey F A, Stackebrandt E
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
Microbiology (Reading). 1996 Oct;142 ( Pt 10):2863-70. doi: 10.1099/13500872-142-10-2863.
A 16S rDNA based molecular ecological study was performed on a sample taken from a peat bog in Germany. Total DNA was extracted by directly lysing micro-organisms in the peat matrix. The 5' 1400 nucleotides of the bacterial 16S rDNA were amplified using conserved bacterial PCR primers. A clone library was generated by blunt-end cloning and 262 16S rDNA clones were analysed. Of these, 37 were located in the Gram-positive phylum, as determined by hybridization to an oligonucleotide probe specific for Gram-positive bacteria. Analysis of 17 of these clones by sequence analysis and their comparison with published sequences representing all of the main bacterial phyla indicated their membership of the actinomycete line of descent. These peat clones were found to represent three novel lineages, two of which appear to be related to the species Acidimicrobium ferrooxidans, and 'Candidatus Microthrix parvicella'. Clone sequences of the third group are phylogenetically related to Rubrobacter radiotolerans. Comparison with short 16S rDNA clone sequences obtained from DNA isolated from a geothermally heated soil in New Zealand, and from DNA isolated from soil in Australia, Japan and Finland and marine environments from the Atlantic and the Pacific Oceans, suggests that members of these three groups occur in very different environments across the world.
对取自德国泥炭沼泽的一个样本进行了基于16S rDNA的分子生态学研究。通过直接裂解泥炭基质中的微生物来提取总DNA。使用保守的细菌PCR引物扩增细菌16S rDNA的5'端1400个核苷酸。通过平端克隆生成克隆文库，并分析了262个16S rDNA克隆。其中，37个位于革兰氏阳性菌门，这是通过与革兰氏阳性菌特异性寡核苷酸探针杂交确定的。通过序列分析对其中17个克隆进行分析，并将它们与代表所有主要细菌门的已发表序列进行比较，表明它们属于放线菌谱系。发现这些泥炭克隆代表三个新谱系，其中两个似乎与嗜酸氧化亚铁硫杆菌和“嗜酸小丝菌属”物种有关。第三组的克隆序列在系统发育上与耐辐射栖热菌相关。与从新西兰地热加热土壤中分离的DNA、从澳大利亚、日本和芬兰的土壤以及大西洋和太平洋海洋环境中分离的DNA获得的短16S rDNA克隆序列进行比较，表明这三组成员存在于世界各地非常不同的环境中。
