Pullen R H, Fatmi A A
Analytical Research and Development, Solvay Pharmaceuticals, Marietta, GA 30062.
J Chromatogr. 1992 Feb 7;574(1):101-7. doi: 10.1016/0378-4347(92)80103-w.
Fluvoxamine and nortriptyline, the assay internal standard, were extracted from plasma with ethyl acetate, then reacted with dansyl chloride. The derivatives were quantitated by isocratic reversed-phase high-performance liquid chromatography with fluorescence detection. The assay calibration range for fluvoxamine was 10-1000 ng/ml using a 1-ml plasma sample. Pooled plasma quality control sample relative recoveries at 25 and 250 ng/ml were 103 and 105%, respectively. Estimates of quality control inter-day precision during validation were less than or equal to 3% relative standard deviation. The assay was cross-validated with a gas chromatographic method and has been employed in therapeutic drug level monitoring.
氟伏沙明和作为测定内标的去甲替林用乙酸乙酯从血浆中萃取，然后与丹磺酰氯反应。衍生物通过等度反相高效液相色谱法结合荧光检测进行定量。使用1 ml血浆样本时，氟伏沙明的测定校准范围为10 - 1000 ng/ml。25 ng/ml和250 ng/ml的合并血浆质量控制样品相对回收率分别为103%和105%。验证期间质量控制日间精密度估计值的相对标准偏差小于或等于3%。该测定法与气相色谱法进行了交叉验证，并已用于治疗药物浓度监测。
