Van der Meersch-Mougeot V, Diquet B
Département de Pharmacologie Clinique, Groupe Hospitalier Pitié-Salpétrière, Paris, France.
J Chromatogr. 1991 Jul 5;567(2):441-9. doi: 10.1016/0378-4347(91)80150-b.
A normal-phase column liquid chromatographic assay for fluvoxamine, a recently introduced atypical antidepressant, has been developed. Prior to analysis, aliquots of alkalinized plasma were extracted with n-hexane. These extracts were then injected into a 5-microns Resolve spherical silica column. Fluvoxamine and the internal standard, clovoxamine, were detected at a wavelength of 254 nm. Standard curves were linear over the concentration range 2-400 ng/ml. Acceptable coefficients of variation were obtained for both within-run and day-to-day studies. The estimated limit of detection is 0.5 ng/ml, and selected drugs checked for coadministration show no analytical interference. This assay was used to evaluate the pharmacokinetics of fluvoxamine in rats.
已开发出一种用于氟伏沙明（一种最近推出的非典型抗抑郁药）的正相柱液相色谱分析法。分析前，取碱化血浆等分试样用正己烷萃取。然后将这些萃取物注入一根5微米的Resolve球形硅胶柱。在254纳米波长处检测氟伏沙明和内标氯伏沙明。标准曲线在2 - 400纳克/毫升的浓度范围内呈线性。批内和日间研究均获得了可接受的变异系数。估计检测限为0.5纳克/毫升，对合用的选定药物进行检查未发现分析干扰。该分析法用于评估氟伏沙明在大鼠体内的药代动力学。
