血浆中华法林的灵敏立体特异性测定：使用(-)-(1S,2R,4R)-内-1,4,5,6,7,7-六氯双环[2.2.1]-庚-5-烯-2-羧酸的非对映异构体酯进行反相高效液相色谱分离

Sensitive stereospecific assay of warfarin in plasma: reversed-phase high-performance liquid chromatographic separation using diastereoisomeric esters of (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]-hept-5- ene-2-carboxylic acid.

作者信息

Carter S R, Duke C C, Cutler D J, Holder G M

机构信息

Department of Pharmacy, University of Sydney, N.S.W., Australia.

出版信息

J Chromatogr. 1992 Feb 7;574(1):77-83. doi: 10.1016/0378-4347(92)80100-5.

DOI:10.1016/0378-4347(92)80100-5

PMID:1629291

Abstract

A stereospecific reversed-phase high-performance liquid chromatographic (HPLC) assay for warfarin in plasma has been developed. The assay involves a rapid, simple clean-up procedure which separates warfarin from plasma constituents and warfarin metabolites. Warfarin enantiomers were assayed as their (-)-(1S,2R,4R)-endo-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene -2- carboxylic acid diastereoisomeric esters by reversed-phase HPLC. Excellent resolution of the diastereoisomers was achieved in less than 10 min. Sensitivity of the assay was approximately 5-10 ng/ml for each isomer.

摘要

已开发出一种用于测定血浆中华法林的立体特异性反相高效液相色谱（HPLC）分析法。该分析法涉及一种快速、简单的净化程序，可将华法林与血浆成分及华法林代谢物分离。通过反相HPLC将华法林对映体作为其（-）-（1S，2R，4R）-内-1，4，5，6，7，7-六氯双环[2.2.1]庚-5-烯-2-羧酸非对映异构体酯进行测定。在不到10分钟的时间内实现了非对映异构体的出色分离。该分析法对每种异构体的灵敏度约为5-10 ng/ml。