Cai W M, Hatton J, Pettigrew L C, Dempsey R J, Chandler M H
Division of Pharmacy Practice and Science, College of Pharmacy, University of Kentucky, Lexington.
Ther Drug Monit. 1994 Oct;16(5):509-12. doi: 10.1097/00007691-199410000-00012.
A simplified method for direct determination of warfarin enantiomers by high-pressure liquid chromatography with fluorescence detection has been developed. This method involves solid phase extraction of warfarin in plasma, precolumn derivatization to form diastereoisomeric esters, and post-column reaction to discriminate each enantiomer separately. Ultrafiltration was employed in the separation of unbound warfarin enantiomers. Twelve plasma samples from six stroke patients taking warfarin regularly were analyzed. The average concentration of total warfarin was 0.47 +/- 0.17 mg/L for the S-isomer and 0.69 +/- 0.18 mg/L for the R-isomer. The average protein binding was 99.67 +/- 0.33% for S-warfarin and 99.44 +/- 0.33% for R-warfarin. This methodology provides a quick and reliable technique for determining enantiomeric protein binding of warfarin in clinical settings.
已开发出一种通过高压液相色谱-荧光检测直接测定华法林对映体的简化方法。该方法包括血浆中华法林的固相萃取、柱前衍生化以形成非对映异构体酯，以及柱后反应以分别鉴别每种对映体。在游离华法林对映体的分离中采用了超滤法。对6名定期服用华法林的中风患者的12份血浆样本进行了分析。S-异构体的总华法林平均浓度为0.47±0.17mg/L，R-异构体为0.69±0.18mg/L。S-华法林的平均蛋白结合率为99.67±0.33%，R-华法林为99.44±0.33%。该方法为在临床环境中测定华法林对映体的蛋白结合提供了一种快速可靠的技术。
