Ikeda M, Kusaka T
Department of Biochemistry, Kawasaki Medical School, Okayama, Japan.
J Chromatogr. 1992 Mar 27;575(2):197-205. doi: 10.1016/0378-4347(92)80146-h.
A useful method for analyzing fatty acids by liquid chromatography-mass spectrometry with an atmospheric-pressure chemical-ionization interface system has been developed. The sensitivity of six kinds of palmitamide derivatives monitored by a single ion of [M+H]+ was, in decreasing order: N-n-propylamide greater than anilide greater than N,N-diethylamide, amide greater than N,N-diphenylamide greater than N-1-naphthylamide. Individual fatty acids were identified from a mixture of amide derivatives of authentic fatty acids from C16:0 to C30:0 on a mass chromatogram. This method was used to detect both hydroxy and non-hydroxy fatty acids. Many kinds of fatty acid, including hydroxy fatty acids of the rat brain, were detected in a single run.
已开发出一种利用具有大气压化学电离接口系统的液相色谱 - 质谱联用技术分析脂肪酸的有效方法。通过[M + H]+单离子监测的六种棕榈酰胺衍生物的灵敏度由高到低依次为：N - 正丙酰胺＞苯胺＞N，N - 二乙酰胺＞酰胺＞N，N - 二苯酰胺＞N - 1 - 萘酰胺。在质量色谱图上，从C16:0至C30:0的真实脂肪酸酰胺衍生物混合物中鉴定出了各个脂肪酸。该方法用于检测羟基脂肪酸和非羟基脂肪酸。单次运行即可检测出多种脂肪酸，包括大鼠脑中的羟基脂肪酸。
