Kusaka T, Ikeda M, Nakano H, Numajiri Y
Biochemistry Department, Kawasaki Medical School, Okayama.
J Biochem. 1988 Oct;104(4):495-7. doi: 10.1093/oxfordjournals.jbchem.a122497.
The mass spectra of a series of saturated (C16:0-C30:0) and unsaturated (C16:1, C18:1, C18:2, C18:3) fatty acids have been recorded as their anilides using liquid chromatography/mass spectrometry with the atmospheric-pressure-ionization interface system. The spectra show an intense peak for (molecule + H)+ ion in each case. The liquid chromatographic/mass spectrometric separation was performed on a reverse phase column using a solvent system of methanol alone or methanol + 2-propanol. This method seemed promising for application to both qualitative and quantitative micro-analysis of fatty acids including very long chain fatty acids.
使用带有大气压电离接口系统的液相色谱/质谱联用仪，记录了一系列饱和（C16:0 - C30:0）和不饱和（C16:1、C18:1、C18:2、C18:3）脂肪酸的苯胺化物的质谱。在每种情况下，光谱都显示出（分子 + H）+离子的强峰。液相色谱/质谱分离在反相柱上进行，使用单独的甲醇或甲醇 + 2 - 丙醇的溶剂系统。该方法似乎有望应用于脂肪酸（包括极长链脂肪酸）的定性和定量微量分析。
