高效液相色谱法同时测定人血浆中咖啡因及其主要去甲基代谢物

Simultaneous determination of caffeine and its primary demethylated metabolites in human plasma by high-performance liquid chromatography.

作者信息

Tanaka E

机构信息

Institute of Community Medicine, University of Tsukuba, Ibaraki-ken, Japan.

出版信息

J Chromatogr. 1992 Mar 27;575(2):311-4. doi: 10.1016/0378-4347(92)80163-k.

DOI:10.1016/0378-4347(92)80163-k

PMID:1629311

Abstract

An improved high-performance liquid chromatographic method for the simultaneous determination of caffeine and its three primary metabolites (theophylline, theobromine and paraxanthine) in human plasma is described. The four substances were separated on a reversed-phase column (5 microns TSK gel ODS-80TM, 150 mm x 4.6 mm I.D.) by use of the mobile phase methanol-0.1 M NaH2PO4 (30:70, v/v) with a flow-rate of 0.8 ml/min. Absorbance was monitored at 274 nm. The detection limit was 5 ng/ml for theobromine and caffeine and 10 ng/ml for paraxanthine and theophylline. The linearity and reproducibility were sufficient for drug monitoring of caffeine and its primary methylxanthines.

摘要

本文描述了一种改进的高效液相色谱法，用于同时测定人血浆中咖啡因及其三种主要代谢物（茶碱、可可碱和副黄嘌呤）。这四种物质在反相柱（5微米TSK凝胶ODS - 80TM，内径150毫米×4.6毫米）上分离，流动相为甲醇 - 0.1M磷酸二氢钠（30:70，v/v），流速为0.8毫升/分钟。在274纳米处监测吸光度。可可碱和咖啡因的检测限为5纳克/毫升，副黄嘌呤和茶碱的检测限为10纳克/毫升。该方法的线性和重现性足以用于咖啡因及其主要甲基黄嘌呤的药物监测。