Jenzsch Marco, Simutis Rimvydas, Luebbert Andreas
Institute of Bioengineering, Martin-Luther University, Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany.
J Biotechnol. 2006 Apr 20;122(4):483-93. doi: 10.1016/j.jbiotec.2005.09.013. Epub 2005 Nov 15.
Generic model control is shown to be a powerful tool for keeping a microbial cultivation process close to its predetermined (optimized) control profile. This is demonstrated at the example of the green fluorescent protein expressed in genetically modified Escherichia coli host cells. It is shown that the process can be run very closely to a predefined complex profile of the specific cell growth rate mu(t). Controlling the experiments at many different growth conditions is a straightforward way of effectively collecting the data necessary for optimization of recombinant protein production systems. Although the process dynamics is rather complex, the model for the controller can be kept quite simple. The control technique, used here for specific growth rate control, is quite universal and can be applied for different biotechnological processes as well.
通用模型控制被证明是一种强大的工具，可使微生物培养过程接近其预定（优化）控制曲线。以在基因改造的大肠杆菌宿主细胞中表达的绿色荧光蛋白为例进行了说明。结果表明，该过程可以非常接近特定细胞生长速率μ(t)的预定义复杂曲线运行。在许多不同生长条件下控制实验是有效收集重组蛋白生产系统优化所需数据的直接方法。尽管过程动力学相当复杂，但控制器的模型可以保持相当简单。这里用于特定生长速率控制的控制技术非常通用，也可应用于不同的生物技术过程。
