Ayadi Imen, Ammar-Keskes Leila, Rebai Ahmed
Bioinformatics Unit, Centre of Biotechnology of Sfax, PB K 3038 Sfax, Tunisia.
Forensic Sci Int. 2006 Dec 20;164(2-3):249-53. doi: 10.1016/j.forsciint.2005.10.006. Epub 2005 Nov 15.
Nine Y-STR loci from the "minimal haplotype" (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393) included in Y-STR Haplotype Reference Databases (YHRD) with 4 additional Y-STRs (DYS436, DYS437, DYS438, DYS439) were analyzed by PCR using duplex and Y-PLEX 12 kit, followed by automatic genotyping in a sample of 105 Tunisian males originating from Sfax region (south Tunisia). Allelic frequencies and gene diversities for each Y-STR locus were determined. The high haplotype diversity (0.9932) and discrimination capacity (0.7714) show the usefulness of these loci for human identification in forensic studies and paternity tests in Tunisia. The most common haplotype was shared by 4.7% (5 individuals) of the sample was only found in samples from the Tunisian population reported in YHRD. One private allele for DYS392 (allele 17) was discovered and duplications were observed for five loci (DYS19, DYS389I, DYS393, DYS437 and DYS439).
对Y染色体短串联重复序列单倍型参考数据库(YHRD)中包含的“最小单倍型”(DYS19、DYS385a/b、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393)中的9个Y-STR基因座,以及另外4个Y-STR基因座(DYS436、DYS437、DYS438、DYS439),采用双重PCR和Y-PLEX 12试剂盒进行分析,随后对来自突尼斯斯法克斯地区(突尼斯南部)的105名突尼斯男性样本进行自动基因分型。确定了每个Y-STR基因座的等位基因频率和基因多样性。高单倍型多样性(0.9932)和鉴别能力(0.7714)表明这些基因座在突尼斯的法医研究和亲子鉴定中的人类身份鉴定方面具有实用性。最常见的单倍型由4.7%(5人)的样本共享,仅在YHRD报告的突尼斯人群样本中发现。发现了一个DYS392的私有等位基因(等位基因17),并观察到五个基因座(DYS19、DYS389I、DYS393、DYS437和DYS439)存在重复现象。