Zhang Jiancheng, Ding Yi, Zhou Zongguang, Li Hongguang, Zhou Bin
Lab. for Division of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Oct;22(5):1024-6, 1044.
The relationship between Human papillomavirus (HPV) 16 infection and the natural course of colorectal adenocarcinoma has not been fully defined. In this study, the HPV 16 E7 DNA was detected in 82 patients with primary colorectal adenocarcinoma to study the relationship between HPV 16 infection and colorectal carcinoma. Samples were taken from both the tumors and the adjacent normal mucosa in the same patient. Polymerase chain reaction (PCR) was used to amplify the HPV16 E7 DNA fragment. The PCR products were gel-purified and sequenced for HPV genotyping. DNA sequence analysis indicated that PCR product was 297 bp. It was the equivalent of 562-858th pairs in the HPV 16 primitive sequences. Our results showed HPV16 E7 DNA expression was significantly higher in colorectal carcinoma (42/82) than in adjacent normal mucosa (4/82). The correlation was found between HPV16 E7 expression and tumor's location; the positive rate was 18.18% in the ascending colon carcinoma group and 64.10% in the rectal carcinoma group. High HPV16 E7 expression was also associated with lower Dukes stage (P < 0.01). These results indicated that there was correlation between colorectal adenocarcinoma and HPV 16 infection. HPV16 infection was relatively higher in the colorectal carcinoma and rare in the adjacent normal mucosa. Specimens expressing higher levels of HPV 16 E7 DNA were associated with lower Dukes stage and more distal location.
人乳头瘤病毒（HPV）16感染与结肠直肠癌的自然病程之间的关系尚未完全明确。在本研究中，检测了82例原发性结肠直肠癌患者的HPV 16 E7 DNA，以研究HPV 16感染与结肠直肠癌之间的关系。样本取自同一患者的肿瘤组织及其相邻的正常黏膜组织。采用聚合酶链反应（PCR）扩增HPV16 E7 DNA片段。对PCR产物进行凝胶纯化并测序以进行HPV基因分型。DNA序列分析表明PCR产物为297 bp。它相当于HPV 16原始序列中的第562 - 858对碱基。我们的结果显示，HPV16 E7 DNA在结肠直肠癌组织（42/82）中的表达显著高于相邻正常黏膜组织（4/82）。发现HPV16 E7表达与肿瘤位置之间存在相关性；升结肠癌组的阳性率为18.18%，直肠癌组为64.10%。高HPV16 E7表达还与较低的Dukes分期相关（P < 0.01）。这些结果表明结肠直肠癌与HPV 16感染之间存在相关性。HPV16感染在结肠直肠癌中相对较高，而在相邻正常黏膜中较少见。表达较高水平HPV 16 E7 DNA的标本与较低的Dukes分期及更靠近远端的位置相关。
