Yu Hong-Gang, Shun Lai-Bao, Luo He-Sheng, Huang He, Yu Bao-Ping, Yu Jie-Ping, Meier Juris J, Schrader Henning, Bastian Andreas, Schmitz Frank, Schmidt Wolfgang E
Department of Gastroentrerology, Renmin Hospital of Wuhan University, 430060 Wuhan, China.
Int J Colorectal Dis. 2002 Nov;17(6):396-401. doi: 10.1007/s00384-002-0404-9. Epub 2002 May 29.
The fragile histidine triad (FHIT) gene, which is frequently lost in many cancers, has been identified as a candidate tumor suppressor gene at chromosome 3p locus 14.2. Human papillomaviruses (HPVs) are the major cause of cervical carcinoma and have been found to be able to integrate its genes into the chromosome 3 fragile site of cultured cells, deleting a piece of DNA which includes the FHIT gene.
We used nested reverse transcriptase PCR and DNA sequencing to evaluate 32 cases of colorectal adenocarcinoma and matched nearby normal tissues for aberrant transcripts of FHIT and infection of high-risk HPVs.
Aberrant transcripts of the FHIT gene were observed in 34.4% of colorectal adenocarcinoma and in 6.3% of matched nearby normal tissues. The HPV(16) DNA was detected in 21.9% of colorectal adenocarcinomas and 3.1% of matched nearby normal tissues using PCR. Only two cases of colorectal adenocarcinoma contained both aberrant transcripts of FHIT and HPV(16) infection. No HPV(18) infection was detected in the present study.
Our results indicate that alteration of the FHIT gene and HPV(16) infection are important genetic events associated with colorectal adenocarcinoma. However, there is no correlation between FHIT abnormalities, clinicopathological features, and HPV(16) infection in colorectal adenocarcinoma.
脆性组氨酸三联体（FHIT）基因在许多癌症中经常缺失，已被确定为位于染色体3p 14.2位点的候选肿瘤抑制基因。人乳头瘤病毒（HPV）是宫颈癌的主要病因，并且已发现其能够将自身基因整合到培养细胞的染色体3脆性位点，缺失一段包含FHIT基因的DNA。
我们使用巢式逆转录PCR和DNA测序来评估32例结直肠腺癌及其配对的邻近正常组织中FHIT异常转录本和高危HPV感染情况。
在34.4%的结直肠腺癌和6.3%的配对邻近正常组织中观察到FHIT基因的异常转录本。使用PCR在21.9%的结直肠腺癌和3.1%的配对邻近正常组织中检测到HPV(16) DNA。仅2例结直肠腺癌同时存在FHIT异常转录本和HPV(16)感染。本研究未检测到HPV(18)感染。
我们的结果表明，FHIT基因改变和HPV(16)感染是与结直肠腺癌相关的重要遗传事件。然而，结直肠腺癌中FHIT异常、临床病理特征与HPV(16)感染之间无相关性。
