Visaprom Sutida, Chindamporn Ariya, Chantratita Wasun, Bhattarakosol Parvapan
Inter-department of Medical Microbiology, Faculty of Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2005 Jul;36(4):910-6.
Whole genomic polymorphisms for 20 HSV-1 and 20 HSV-2 isolates from Thai patients were analyzed by means of Restriction Fragment Length Polymorphism (RFLP) analysis using 4 restriction endonucleases: BamHI, Kpnl, HindIII, and EcoRI. Variations in cleavage sites among the HSV-1 and HSV-2 isolates were compared to the cleavage patterns of standard HSV-1 strain KOS and HSV-2 strain Baylor 186. Although 70% of HSV-1 isolates with BamHI digestion, 50% with Kpnl, 75% with HindIII and 70% with EcoRI digestion were found to be similar to the standard HSV-1 (KOS) pattern, new BamHI restriction sites were detected in some HSV-1 isolates. For HSV-2 isolates, 85% had the same pattern as the standard HSV-2 (Baylor 186) after digestion with BamHI, HindIII, and EcoRI. No difference was observed with Kpnl digestion. When the patterns from the 4 enzymes were combined, HSV-1 isolates showed more divergence than the HSV-2 isolates. HSV-1 isolates found in both non-genital and genital lesions had more variety than the HSV-2 isolates. This suggests that intratypic variations in HSV-2 are fewer than in HSV-1.
利用4种限制性内切酶（BamHI、Kpnl、HindIII和EcoRI），通过限制性片段长度多态性（RFLP）分析，对来自泰国患者的20株单纯疱疹病毒1型（HSV-1）和20株单纯疱疹病毒2型（HSV-2）分离株的全基因组多态性进行了分析。将HSV-1和HSV-2分离株切割位点的变异与标准HSV-1毒株KOS和HSV-2毒株Baylor 186的切割模式进行了比较。虽然发现70%经BamHI消化的HSV-1分离株、50%经Kpnl消化的、75%经HindIII消化的以及70%经EcoRI消化的分离株与标准HSV-1（KOS）模式相似，但在一些HSV-1分离株中检测到了新的BamHI限制性位点。对于HSV-2分离株，经BamHI、HindIII和EcoRI消化后，85%具有与标准HSV-2（Baylor 186）相同的模式。Kpnl消化未观察到差异。当将4种酶的模式组合时，HSV-1分离株比HSV-2分离株表现出更多的差异。在非生殖器和生殖器病变中均发现的HSV-1分离株比HSV-2分离株具有更多样性。这表明HSV-2的型内变异比HSV-1少。
