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利用限制酶对临床1型单纯疱疹病毒分离株进行基因分型。

Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.

作者信息

Norberg Peter, Bergström Tomas, Liljeqvist Jan-Ake

机构信息

Department of Clinical Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden.

出版信息

J Clin Microbiol. 2006 Dec;44(12):4511-4. doi: 10.1128/JCM.00421-06. Epub 2006 Oct 11.

DOI:10.1128/JCM.00421-06
PMID:17035491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1698414/
Abstract

Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.

摘要

最近，基于临床分离株和实验室菌株的DNA序列信息及系统发育分析，鉴定出了三种不同基因型的临床1型单纯疱疹病毒（HSV-1）分离株。我们利用编码糖蛋白G和I的基因内的单核苷酸多态性，通过PCR和限制性内切酶切割进行快速基因型分类。该方法适用于临床HSV-1分离株的大规模基因分型以及重组体的检测。

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本文引用的文献

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Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2.通过针对单纯疱疹病毒1型和2型同源gB片段的TaqMan聚合酶链反应检测和分型黏膜皮肤样本中的单纯疱疹病毒
J Clin Microbiol. 2005 May;43(5):2058-64. doi: 10.1128/JCM.43.5.2058-2064.2005.
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Neonatal herpes: what have we learned.新生儿疱疹：我们学到了什么。
Semin Pediatr Infect Dis. 2005 Jan;16(1):7-16. doi: 10.1053/j.spid.2004.09.006.
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Human herpes simplex virus keratitis: the pathogenesis revisited.人类单纯疱疹病毒性角膜炎：发病机制再探讨。
Ocul Immunol Inflamm. 2004 Dec;12(4):255-85. doi: 10.1080/092739490500363.
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Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses.对临床1型单纯疱疹病毒分离株进行的系统发育分析确定了三个基因群和重组病毒。
J Virol. 2004 Oct;78(19):10755-64. doi: 10.1128/JVI.78.19.10755-10764.2004.
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Neonatal herpes simplex virus infection.新生儿单纯疱疹病毒感染
Curr Opin Infect Dis. 2004 Jun;17(3):243-6. doi: 10.1097/00001432-200406000-00012.
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High recombination rate in herpes simplex virus type 1 natural populations suggests significant co-infection.单纯疱疹病毒1型自然群体中的高重组率表明存在显著的共感染。
Infect Genet Evol. 2004 Jun;4(2):115-23. doi: 10.1016/j.meegid.2004.01.009.
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Genotyping of herpes simplex virus type 1 strains isolated from ocular materials of patients with herpetic keratitis.从单纯疱疹病毒性角膜炎患者眼部材料中分离出的1型单纯疱疹病毒毒株的基因分型。
J Med Virol. 2003 Sep;71(1):75-81. doi: 10.1002/jmv.10466.
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A herpes simplex virus 1 recombinant lacking the glycoprotein G coding sequences is defective in entry through apical surfaces of polarized epithelial cells in culture and in vivo.一种缺失糖蛋白G编码序列的单纯疱疹病毒1型重组体，在体外培养和体内通过极化上皮细胞的顶端表面进入细胞的过程中存在缺陷。
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1818-22. doi: 10.1073/pnas.020510297.
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Reactivation of herpes simplex virus type 1 in patients with Bell's palsy.贝尔麻痹患者中单纯疱疹病毒1型的再激活
J Med Virol. 1998 Mar;54(3):162-6. doi: 10.1002/(sici)1096-9071(199803)54:3<162::aid-jmv3>3.0.co;2-3.
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Quantitative analysis of genomic polymorphism of herpes simplex virus type 1 strains from six countries: studies of molecular evolution and molecular epidemiology of the virus.六个国家1型单纯疱疹病毒株基因组多态性的定量分析：病毒的分子进化与分子流行病学研究
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