利用限制酶对临床1型单纯疱疹病毒分离株进行基因分型。
Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.
作者信息
Norberg Peter, Bergström Tomas, Liljeqvist Jan-Ake
机构信息
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden.
出版信息
J Clin Microbiol. 2006 Dec;44(12):4511-4. doi: 10.1128/JCM.00421-06. Epub 2006 Oct 11.
Abstract
Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.
摘要
最近，基于临床分离株和实验室菌株的DNA序列信息及系统发育分析，鉴定出了三种不同基因型的临床1型单纯疱疹病毒（HSV-1）分离株。我们利用编码糖蛋白G和I的基因内的单核苷酸多态性，通过PCR和限制性内切酶切割进行快速基因型分类。该方法适用于临床HSV-1分离株的大规模基因分型以及重组体的检测。
相似文献
1
Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.利用限制酶对临床1型单纯疱疹病毒分离株进行基因分型。
J Clin Microbiol. 2006 Dec;44(12):4511-4. doi: 10.1128/JCM.00421-06. Epub 2006 Oct 11.
2
A 10-year molecular survey of herpes simplex virus type 1 in Germany demonstrates a stable and high prevalence of genotypes A and B.一项针对德国1型单纯疱疹病毒的为期10年的分子调查显示，A和B基因型具有稳定且较高的流行率。
J Clin Virol. 2009 Mar;44(3):235-7. doi: 10.1016/j.jcv.2008.12.016. Epub 2009 Jan 30.
3
Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis.单纯疱疹病毒1型糖蛋白G（gG）和gI基因型在疱疹性角膜炎患者中的流行情况。
Br J Ophthalmol. 2008 Sep;92(9):1195-200. doi: 10.1136/bjo.2007.136044. Epub 2008 Jul 10.
4
Herpes simplex virus genotyping: multiple optional PCR-based RFLP systems and a non-isotopic single-strand conformation polymorphism method.单纯疱疹病毒基因分型：多种基于聚合酶链反应的限制性片段长度多态性可选系统及一种非同位素单链构象多态性方法。
J Virol Methods. 2004 Jun 1;118(1):73-6. doi: 10.1016/j.jviromet.2004.01.013.
5
Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with different herpetic diseases during the last four decades.过去四十年间不同疱疹性疾病患者中单纯疱疹病毒 1 型糖蛋白 G（gG）和 gI 基因型的流行情况。
J Med Virol. 2012 Apr;84(4):651-6. doi: 10.1002/jmv.23223.
6
A duplex PCR assay for detection and genotyping of Herpes simplex virus in cerebrospinal fluid.一种用于检测脑脊液中单纯疱疹病毒并进行基因分型的双重聚合酶链反应检测法。
Mol Cell Probes. 1999 Aug;13(4):309-14. doi: 10.1006/mcpr.1999.0253.
7
Detection of differences in genomic profiles between herpes simplex virus type 1 isolates sequentially separated from the saliva of the same individual.对从同一个体唾液中相继分离出的1型单纯疱疹病毒分离株之间基因组图谱差异的检测。
J Clin Virol. 2007 Aug;39(4):266-70. doi: 10.1016/j.jcv.2007.05.012. Epub 2007 Jul 5.
8
Comparison of two methods of PCR followed by enzymatic restriction digestion for detection and typing of herpes simplex viruses isolated from patients with mucocutaneous or cutaneous lesions.两种聚合酶链反应（PCR）方法随后进行酶切消化用于检测和分型从患有黏膜皮肤或皮肤病变患者中分离出的单纯疱疹病毒的比较。
Rev Latinoam Microbiol. 2005 Jul-Dec;47(3-4):76-81.
9
Molecular characterization of clinical isolates of herpes simplex virus type 1 collected in a tertiary-care hospital in Dublin, Ireland.爱尔兰都柏林一家三级保健医院采集的单纯疱疹病毒 1 型临床分离株的分子特征。
J Med Virol. 2013 May;85(5):839-44. doi: 10.1002/jmv.23541.
10
A 12-year molecular survey of clinical herpes simplex virus type 2 isolates demonstrates the circulation of clade A and B strains in Germany.一项针对临床单纯疱疹病毒 2 型分离株的 12 年分子调查表明，A 株和 B 株在德国流行。
J Clin Virol. 2010 Jul;48(3):208-11. doi: 10.1016/j.jcv.2010.04.009. Epub 2010 May 21.
引用本文的文献
1
Oncoviruses in the Oral Cavity: Recent Advances in Understanding Viral Infections and Tumorigenesis.口腔中的肿瘤病毒：病毒感染与肿瘤发生认识的最新进展
Int J Mol Sci. 2025 Jul 13;26(14):6721. doi: 10.3390/ijms26146721.
2
Ancient herpes simplex 1 genomes reveal recent viral structure in Eurasia.古代单纯疱疹病毒1型基因组揭示了欧亚大陆近期的病毒结构。
Sci Adv. 2022 Jul 29;8(30):eabo4435. doi: 10.1126/sciadv.abo4435. Epub 2022 Jul 27.
3
Callitrichine gammaherpesvirus 3 and Human alphaherpesvirus 1 in New World Primate negative for yellow fever virus in Rio de Janeiro, Brazil.巴西里约热内卢无黄热病毒的新世界灵长类动物中存在 Callitrichine gammaherpesvirus 3 和人类 alphaherpesvirus 1。
Mem Inst Oswaldo Cruz. 2022 Apr 11;117:e210258. doi: 10.1590/0074-02760210258. eCollection 2022.
4
Varicella-Zoster Virus-Genetics, Molecular Evolution and Recombination.水痘带状疱疹病毒的遗传学、分子进化与重组。
Curr Top Microbiol Immunol. 2023;438:1-23. doi: 10.1007/82_2021_238.
5
Zebra Alphaherpesviruses (EHV-1 and EHV-9): Genetic Diversity, Latency and Co-Infections.斑马α疱疹病毒（EHV-1和EHV-9）：遗传多样性、潜伏感染与共感染
Viruses. 2016 Sep 20;8(9):262. doi: 10.3390/v8090262.
6
History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains.单纯疱疹病毒1型KOS和KOS1.1亚株的历史及基因组序列分析
Virology. 2016 Jan;487:215-21. doi: 10.1016/j.virol.2015.09.026. Epub 2015 Nov 5.
7
Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil.巴西里约热内卢免疫功能低下患者中1型单纯疱疹病毒分离株的基因特征分析。
PLoS One. 2015 Sep 25;10(9):e0136825. doi: 10.1371/journal.pone.0136825. eCollection 2015.
8
Genetic variability in the region encompassing reiteration VII of herpes simplex virus type 1, including deletions and multiplications related to recombination between direct repeats.1型单纯疱疹病毒重复序列VII区域的基因变异性，包括与正向重复序列之间重组相关的缺失和倍增。
Springerplus. 2015 Apr 30;4:200. doi: 10.1186/s40064-015-0990-y. eCollection 2015.
9
Recombination of Globally Circulating Varicella-Zoster Virus.全球流行的水痘-带状疱疹病毒的重组
J Virol. 2015 Jul;89(14):7133-46. doi: 10.1128/JVI.00437-15.
10
Rad51 and Rad52 are involved in homologous recombination of replicating herpes simplex virus DNA.Rad51和Rad52参与复制型单纯疱疹病毒DNA的同源重组。
PLoS One. 2014 Nov 3;9(11):e111584. doi: 10.1371/journal.pone.0111584. eCollection 2014.
本文引用的文献
1
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2.通过针对单纯疱疹病毒1型和2型同源gB片段的TaqMan聚合酶链反应检测和分型黏膜皮肤样本中的单纯疱疹病毒
J Clin Microbiol. 2005 May;43(5):2058-64. doi: 10.1128/JCM.43.5.2058-2064.2005.
2
Neonatal herpes: what have we learned.新生儿疱疹：我们学到了什么。
Semin Pediatr Infect Dis. 2005 Jan;16(1):7-16. doi: 10.1053/j.spid.2004.09.006.
3
Human herpes simplex virus keratitis: the pathogenesis revisited.人类单纯疱疹病毒性角膜炎：发病机制再探讨。
Ocul Immunol Inflamm. 2004 Dec;12(4):255-85. doi: 10.1080/092739490500363.
4
Phylogenetic analysis of clinical herpes simplex virus type 1 isolates identified three genetic groups and recombinant viruses.对临床1型单纯疱疹病毒分离株进行的系统发育分析确定了三个基因群和重组病毒。
J Virol. 2004 Oct;78(19):10755-64. doi: 10.1128/JVI.78.19.10755-10764.2004.
5
Neonatal herpes simplex virus infection.新生儿单纯疱疹病毒感染
Curr Opin Infect Dis. 2004 Jun;17(3):243-6. doi: 10.1097/00001432-200406000-00012.
6
High recombination rate in herpes simplex virus type 1 natural populations suggests significant co-infection.单纯疱疹病毒1型自然群体中的高重组率表明存在显著的共感染。
Infect Genet Evol. 2004 Jun;4(2):115-23. doi: 10.1016/j.meegid.2004.01.009.
7
Genotyping of herpes simplex virus type 1 strains isolated from ocular materials of patients with herpetic keratitis.从单纯疱疹病毒性角膜炎患者眼部材料中分离出的1型单纯疱疹病毒毒株的基因分型。
J Med Virol. 2003 Sep;71(1):75-81. doi: 10.1002/jmv.10466.
8
A herpes simplex virus 1 recombinant lacking the glycoprotein G coding sequences is defective in entry through apical surfaces of polarized epithelial cells in culture and in vivo.一种缺失糖蛋白G编码序列的单纯疱疹病毒1型重组体，在体外培养和体内通过极化上皮细胞的顶端表面进入细胞的过程中存在缺陷。
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1818-22. doi: 10.1073/pnas.020510297.
9
Reactivation of herpes simplex virus type 1 in patients with Bell's palsy.贝尔麻痹患者中单纯疱疹病毒1型的再激活
J Med Virol. 1998 Mar;54(3):162-6. doi: 10.1002/(sici)1096-9071(199803)54:3<162::aid-jmv3>3.0.co;2-3.
10
Quantitative analysis of genomic polymorphism of herpes simplex virus type 1 strains from six countries: studies of molecular evolution and molecular epidemiology of the virus.六个国家1型单纯疱疹病毒株基因组多态性的定量分析：病毒的分子进化与分子流行病学研究
J Gen Virol. 1994 Mar;75 ( Pt 3):513-27. doi: 10.1099/0022-1317-75-3-513.
Zotero 插件