曼谷朱拉隆功国王纪念医院单纯疱疹病毒分离株的阿昔洛韦敏感性
Acyclovir susceptibility of herpes simplex virus isolates at King Chulalongkorn Memorial Hospital, Bangkok.
作者信息
Sangdara Ajchariyarat, Bhattarakosol Parvapan
机构信息
Inter-department of Medical Microbiology, Faculty ofGraduate School, Chulalongkorn University, Bangkok, Thailand.
出版信息
J Med Assoc Thai. 2008 Jun;91(6):908-12.
OBJECTIVE
To determine the ACV susceptibility in Thai HSV clinical isolates.
MATERIAL AND METHOD
One hundred thirty HSV isolates from the Virology Laboratory Unit, King Chulalongkorn Memorial Hospital, Bangkok Thailand had typing done by immunofluoresent assay using monoclonal antibody specific to either HSV-1 or HSV-2. Their sensitivity to ACV (IC50) was determined by plaque reduction assay.
RESULTS
The IC50 of 77 HSV-1 isolates ranged from 0.07-0.97 microg/ml and that of 53 HSV-2 isolates was 0.13-1.66 microg/ml. The standard HSV-1 (KOS) and HSV-2 (Baylor 186) were included in each run. The mean + standard deviation (SD) of ACV IC50 among HSV-1 and HSV-2 isolates were 0.38 +/- 0.23 and 0.50 +/- 0.32 microg/ml while that of standard HSV-1 and HSV-2 were 0.45 +/- 0.13 and 0.57 +/- 0.04 microg/ml. Statistically significant difference between IC50 of HSV-1 and HSV-2 isolates was indicated (p = 0.02).
CONCLUSION
No ACV(r) HSV has been detected and ACV susceptibility of HSV-2 has more resistance than that of HSV-1.
目的
确定泰国单纯疱疹病毒（HSV）临床分离株对阿昔洛韦（ACV）的敏感性。
材料与方法
从泰国曼谷朱拉隆功国王纪念医院病毒学实验室单元获取130株HSV分离株，使用针对HSV - 1或HSV - 2的单克隆抗体通过免疫荧光测定法进行分型。通过蚀斑减少试验测定它们对ACV的敏感性（IC50）。
结果
77株HSV - 1分离株的IC50范围为0.07 - 0.97微克/毫升，53株HSV - 2分离株的IC50为0.13 - 1.66微克/毫升。每次试验均纳入标准HSV - 1（KOS）和HSV - 2（贝勒186）。HSV - 1和HSV - 2分离株中ACV IC50的平均值 + 标准差（SD）分别为0.38 ± 0.23和0.50 ± 0.32微克/毫升；而标准HSV - 1和HSV - 2的分别为0.45 ± 0.13和0.57 ± 0.04微克/毫升。HSV - 1和HSV - 2分离株的IC50之间存在统计学显著差异（p = 0.02））。
结论
未检测到对阿昔洛韦耐药的HSV，且HSV - 2对阿昔洛韦的敏感性比HSV - 1更低。
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