Mlcková P, Polácek M, Cechová D, Marusková L, Stanková I, Chalupná P, Novotná O, Julák J, Prokesová L
Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czechia.
Folia Microbiol (Praha). 2005;50(3):247-53. doi: 10.1007/BF02931573.
Inactivated Bacillus firmus (BF), G+ nonpathogenic bacterium of the external environment, was coupled to ovalbumin (OVA) and used in immunization experiments as antigen carrier. Balb/c mice were immunized thrice intra-tracheally and intra-nasally with conjugates of OVA and BF. Surprisingly, administration of OVA-BF conjugates inhibited anti-OVA IgG response in both sera and mucosal secretions if compared to an exposure to OVA alone. The suppression of antigen-specific antibody production was accompanied by promotion of TH1 phenotype.
灭活的坚强芽孢杆菌（BF）是一种外部环境中的革兰氏阳性非致病细菌，它与卵清蛋白（OVA）偶联，并用作免疫实验中的抗原载体。将Balb/c小鼠经气管内和鼻内三次免疫接种OVA与BF的偶联物。令人惊讶的是，与单独暴露于OVA相比，OVA-BF偶联物的给药抑制了血清和粘膜分泌物中的抗OVA IgG反应。抗原特异性抗体产生的抑制伴随着TH1表型的促进。
