Prokesová L, Mlcková P, Stanková I, Chloubová A, Novotná V, Ladmanová P, Chalupná P, Mára M
Institute of Clinical Immunology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Immunol Lett. 1998 Dec;64(2-3):161-6. doi: 10.1016/s0165-2478(98)00105-9.
Immunostimulatory properties of B. firmus, a nontoxic, nonpathogenic G + bacterium of external environment, were described previously. Antiinfectious and antitumor activity, macrophage activation and strong polyclonal stimulation of B lymphocytes were proved in human, mice and rats. The adjuvant effect of B. firmus on specific antibody response to ovalbumin in BALB/c mice is the topic of the present study. Against our expectation, B. firmus exerts more suppressive than stimulatory effect on specific antibody response. Formolized B. firmus decreased anti-ovalbumin response after subcutaneous immunization and only slightly increased serum antibodies after intraperitoneal immunization. After mucosal immunization, both oral and rectal, ovalbumin itself did not cause a significant systemic response but induced IgA anti-ovalbumin response in the intestine. B. firmus applied together with ovalbumin increased systemic serum response but absolutely eliminated intestinal response. The rectal route of antigen administration has been found less convenient because of less precise dosing of antigen in this mode of immunization.
先前已描述了坚强芽孢杆菌的免疫刺激特性，它是一种存在于外部环境中的无毒、非致病性革兰氏阳性菌。在人、小鼠和大鼠体内均证实了其抗感染和抗肿瘤活性、巨噬细胞激活以及对B淋巴细胞的强烈多克隆刺激作用。本研究的主题是坚强芽孢杆菌对BALB/c小鼠针对卵清蛋白的特异性抗体反应的佐剂效应。与我们的预期相反，坚强芽孢杆菌对特异性抗体反应的抑制作用大于刺激作用。经甲醛处理的坚强芽孢杆菌在皮下免疫后降低了抗卵清蛋白反应，而在腹腔免疫后仅轻微增加了血清抗体。在黏膜免疫后，无论是口服还是直肠给药，卵清蛋白本身并未引起显著的全身反应，但在肠道中诱导了抗卵清蛋白IgA反应。与卵清蛋白一起应用的坚强芽孢杆菌增加了全身血清反应，但完全消除了肠道反应。由于在这种免疫方式中抗原剂量不太精确，已发现直肠途径给药不太方便。
