Fugaro Orlando J, Fugaro Jessica O, Matis Bruce, Gregory Richard L, Cochran Michael A, Mjör Ivar
San Mateo Branch Clinic, U.S. Navy, Camp Pendelton, CA, USA.
Am J Dent. 2005 Aug;18(4):229-32.
To evaluate the expression of specific neuropeptides associated with inflammation, substance-P (SP) and calcitonin gene-related peptide (CGRP), after night guard vital bleaching with 10% carbamide peroxide gel.
10 patients with four caries free premolars scheduled for orthodontic extraction had these teeth bleached with 10% Opalescence as follows: tooth #28, no treatment (control); #5, 4 days of bleaching; #12, 2 weeks of bleaching; #21, 2 weeks of bleaching, followed by 2 weeks without treatment. All teeth in the four groups were extracted at the same time. Immediately after extraction the teeth were frozen at -197 degrees C in liquid nitrogen. The pulp samples were prepared for enzyme-linked immunoabsorbent assay (ELISA) to determine the concentrations of the SP, CGRP and total protein in the pulp tissue.
The analysis of mean SP and mean CGRP/ng protein/ml resulted in no significant differences among the four groups. It was concluded that the study did not demonstrate an increase in the release of SP and/or CGRP during night guard vital bleaching of teeth with 10% Opalescence.
评估使用10%过氧化脲凝胶进行夜间护齿活髓漂白后，与炎症相关的特定神经肽——P物质（SP）和降钙素基因相关肽（CGRP）的表达情况。
10名计划进行正畸拔牙的患者有四颗无龋前磨牙，用10%皓齿美白凝胶对这些牙齿进行如下漂白：28号牙，不做处理（对照）；5号牙，漂白4天；12号牙，漂白2周；21号牙，漂白2周，然后2周不做处理。四组中的所有牙齿同时拔除。拔牙后立即将牙齿在液氮中于-197℃冷冻。制备牙髓样本用于酶联免疫吸附测定（ELISA），以确定牙髓组织中SP、CGRP和总蛋白的浓度。
四组之间SP均值和CGRP/ng蛋白/ml均值的分析结果无显著差异。得出的结论是，该研究未证明使用10%皓齿美白凝胶进行牙齿夜间护齿活髓漂白期间SP和/或CGRP的释放增加。
