Umino Ayuko, Nikaido Toru, Tsuchiya Satoko, Foxton Richard M, Tagami Junji
Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan.
Am J Dent. 2005 Aug;18(4):245-50.
To analyze in vitro artificial secondary caries inhibition around conventional luting cements and resin cements using a confocal laser-scanning microscope (CLSM).
Box shape cavities (approximately 3 mm long, 4 mm wide, and 1.5 mm deep) were prepared in bovine root dentin. One of five cements: Elite Cement 100 (EL) zinc phosphate cement, HY-Bond Carbo Cement (CA) polycarboxylate cement, Fuji I (FI) glass-ionomer cement, Fuji Luting (FL) GIC-based resin cement and Panavia F (PA) fluoride-releasing resin cement, was placed in the cavity. After polishing, the center of the cement surface was covered with an adhesive to preserve the original cement solubility. The specimens were stored in distilled water at 37 degrees C for 1 week, and stored in an artificial demineralizing solution for 3.5 days. Following this, each specimen was sectioned into two halves, trimmed, and polished. Thickness of inhibition zone, depth of outer lesion and cement solubility around each cement were determined by a CLSM.
Inhibition zones were not seen in EL and CA, while they were found in FI, FL and PA. The depths of the outer lesions of CA and FI were significantly lower than those of EL, FL and PA (P< 0.05). The highest and the second-highest cement solubility were obtained in EL and CA, respectively. FI and FL indicated significantly lower cement solubility than EL and CA. PA tended to show the lowest cement solubility.
使用共聚焦激光扫描显微镜（CLSM）分析传统黏固剂和树脂黏固剂周围的体外人工继发龋抑制情况。
在牛牙根牙本质中制备箱形洞（长约3mm、宽4mm、深1.5mm）。将以下五种黏固剂之一：Elite Cement 100（EL）磷酸锌黏固剂、HY-Bond Carbo Cement（CA）聚羧酸锌黏固剂、Fuji I（FI）玻璃离子黏固剂、Fuji Luting（FL）玻璃离子基树脂黏固剂和Panavia F（PA）含氟树脂黏固剂，置于洞中。抛光后，用黏合剂覆盖黏固剂表面中心以保持原始黏固剂的溶解性。将标本在37℃蒸馏水中储存1周，然后在人工脱矿溶液中储存3.5天。之后，将每个标本切成两半，修整并抛光。通过CLSM测定每种黏固剂周围的抑制区厚度、外层病变深度和黏固剂溶解性。
EL和CA中未见抑制区，而在FI、FL和PA中发现有抑制区。CA和FI的外层病变深度显著低于EL、FL和PA（P<0.05）。EL和CA的黏固剂溶解性分别最高和次高。FI和FL的黏固剂溶解性显著低于EL和CA。PA的黏固剂溶解性往往最低。
