Bakshi Rachit, Mehta Abhishek Kumar, Sharma Ritu, Maiti Souvik, Pasha Santosh, Brahmachari Vani
Dr. B. R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi-110007, India.
Biochem Biophys Res Commun. 2006 Jan 6;339(1):313-20. doi: 10.1016/j.bbrc.2005.10.206. Epub 2005 Nov 15.
The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. Here we report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity. A differential expression of the various domains within this gene is detected in human tissues while a ubiquitous expression is detected in mice. The intranuclear localization is demonstrated using antibodies directed against the DBINO domain of hINO80.
属于依赖DNA的ATP酶SWI2/SNF2家族的蛋白质是染色质重塑复合物的重要成员，这些复合物与基因表达的表观遗传控制有关。我们鉴定出了一个具有推定DNA结合结构域的人类基因，它属于SWI2/SNF2蛋白的INO80亚家族。在此，我们报告了hINO80基因各结构域的克隆、表达及其在依赖DNA的ATP酶活性和DNA结合活性方面的功能活性。该基因内不同结构域在人体组织中存在差异表达，而在小鼠中则检测到普遍表达。利用针对hINO80的DBINO结构域的抗体证实了其核内定位。
