Yuste Rafael
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Nat Methods. 2005 Dec;2(12):902-4. doi: 10.1038/nmeth1205-902.
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyper-resolution microscopies are poised to profoundly transform many fields of biological research.
在过去十年中，荧光显微镜技术经历了复兴。绿色荧光蛋白（GFP）和双光子显微镜的引入，使得对活细胞中蛋白质定位以及活组织的结构与功能进行系统性成像研究成为可能。这些以及其他新的成像方法在生物物理学、神经科学、发育生物学和细胞生物学领域产生了显著影响。荧光团设计、分子生物学工具以及非线性和超分辨率显微镜技术的进一步发展，有望深刻改变生物研究的许多领域。
