McDonald Lucy, Robertson Duncan H L, Hurst Jane L, Beynon Robert J
Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK.
Nat Methods. 2005 Dec;2(12):955-7. doi: 10.1038/nmeth811. Epub 2005 Nov 18.
Bottom-up proteomics is the analysis of peptides derived from single proteins or protein mixtures, and because each protein generates tens of peptides, there is scope for controlled reduction in complexity. We report here a new strategy for selective isolation of the N-terminal peptides of a protein mixture, yielding positionally defined peptides. The method is tolerant of several fragmentation methods, and the databases that must be searched are substantially less complex.
自下而上的蛋白质组学是对源自单一蛋白质或蛋白质混合物的肽段进行分析，并且由于每种蛋白质会产生数十种肽段，因此存在可控降低复杂性的空间。我们在此报告一种用于选择性分离蛋白质混合物N端肽段的新策略，可产生位置明确的肽段。该方法适用于多种碎裂方法，且必须搜索的数据库复杂性显著降低。
