Idilman R, Kuzu I, Erden E, Arat M, Soydan E, Soykan I, Akyol G, Karayalcin S, Akan H, Beksac M
Department of Gastroenterology, Ankara University School of Medicine, Ibn'i Sina Hospital, Sihhiye, Ankara, Turkey.
Bone Marrow Transplant. 2006 Jan;37(2):199-206. doi: 10.1038/sj.bmt.1705214.
The aim of this study was to detect donor-derived hepatocytes and gastrointestinal epithelial cells in recipients of sex-mismatched allogeneic hematopoietic cell transplants, and to assess the effect of tissue injury on the extent of the repopulation. A total of 29 paraffin-embedded biopsy samples were reviewed. Double labeling by immunohistochemistry and fluorescence in situ hybridization was performed. Eighty-nine percent of sex-mismatched samples with histologic evidence of injury demonstrated the presence of donor-derived hepatocytes and gastrointestinal epithelial cells (mean 2.4%). None of the hepatocytes and gastrointestinal epithelial cells in samples obtained from female recipients with female donors showed a Y chromosome signal. The proportion of donor-derived hepatocyte and gastrointestinal epithelial cells in samples with severe graft-versus-host disease was greater than that of samples with mild/moderate graft-versus-host disease (P = 0.09). No relationship between the source of stem cells and the population rate was detected (P > 0.05). We conclude that some recipient hepatocytes and gastrointestinal tract epithelial cells are replaced by donor-derived cells during tissue injury. The severity of tissue injury seems to influence on the extent of this repopulation.
本研究的目的是检测性别不匹配的异基因造血细胞移植受者体内供体来源的肝细胞和胃肠道上皮细胞，并评估组织损伤对细胞再填充程度的影响。共审查了29份石蜡包埋的活检样本。采用免疫组织化学和荧光原位杂交进行双重标记。89%有组织损伤组织学证据的性别不匹配样本显示存在供体来源的肝细胞和胃肠道上皮细胞（平均2.4%）。从女性供体的女性受者获得的样本中，肝细胞和胃肠道上皮细胞均未显示Y染色体信号。重度移植物抗宿主病样本中供体来源的肝细胞和胃肠道上皮细胞比例高于轻度/中度移植物抗宿主病样本（P = 0.09）。未检测到干细胞来源与细胞植入率之间的关系（P > 0.05）。我们得出结论，在组织损伤期间，一些受者的肝细胞和胃肠道上皮细胞被供体来源的细胞所取代。组织损伤的严重程度似乎影响这种细胞再填充的程度。
