Chang Jian-xing, Chen Shuang, Jiang Long-yuan, Ma Li-ping, Chang Rui-ming, Huang Zi-tong
The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2005 Nov;8(6):510-2.
To investigate the changes of the goblet cells in the intestine during the restitution process of the gut barrier after hemorrhagic shock.
Forty-nine Sprague-Dawley rats with body weight of 250-300 g were divided into control group (n=7) and experimental group (n=42). Rats in the experimental group was further divided into 6 groups (n=7 each) according to different time point at 1, 3, 6, 12, 24, and 36 hours after hemorrhagic shock resuscitation. The specimens from ileum tissue were taken to observe the morphological chan ges of the intestinal mucosa. The number of goblet cells was determined by light microscope and/or electron microscope. The contents of trefoil factor family 3 (TFF3) of goblet cells were examined using GC-9A gas chromatographic instrument.
After hemorrhagic shock, mucosal epithelial injury was obvious in the small intestine. Tissue restitution was found after 3 hours, and mostly established after 12 hours. Following tissue restitution,the denuded mucosal surface was covered intensively by goblet cells. The number of goblet cells on the intestinal mucosa was reduced significantly from 243+/- 13 at 1 h to 157+/- 9 at 24 h (r=- 0.910, P< 0.01), and returned to normal level at 36 h. In the experimental group, the content of TFF3 in the intestinal mucosa increased significantly at 12 hours, decreased, but was still higher at 24 hours (t=3.24, P< 0.05).
The goblet cells play a key role in the restitution of intestinal mucosa. High expression of TFF3 may facilitate the intestinal mucosal restitution in the early phase.
探讨失血性休克后肠道屏障恢复过程中肠道杯状细胞的变化。
将49只体重250 - 300 g的Sprague-Dawley大鼠分为对照组（n = 7）和实验组（n = 42）。实验组大鼠根据失血性休克复苏后1、3、6、12、24和36小时的不同时间点进一步分为6组（每组n = 7）。取小肠组织标本观察肠黏膜的形态变化。通过光学显微镜和/或电子显微镜确定杯状细胞的数量。使用GC - 9A气相色谱仪检测杯状细胞三叶因子家族3（TFF3）的含量。
失血性休克后，小肠黏膜上皮损伤明显。3小时后发现组织修复，12小时后基本完成修复。组织修复后，裸露的黏膜表面被杯状细胞密集覆盖。肠黏膜上杯状细胞的数量从1小时时的243±13显著减少至24小时时的157±9（r = - 0.910，P < 0.01），并在36小时时恢复到正常水平。在实验组中，肠黏膜中TFF3的含量在12小时时显著增加，随后下降，但在24小时时仍高于正常水平（t = 3.24，P < 0.05）。
杯状细胞在肠黏膜修复中起关键作用。TFF3的高表达可能在早期促进肠黏膜修复。
