Ikeda Hiroshi, Yang Chao-Long, Tong Jie, Nishimaki Haruaki, Masuda Kenji, Takeo Tomohiro, Kasai Kenji, Itoh Gen
First Department of Pathology, Aichi Medical University, Japan.
Dig Dis Sci. 2002 Mar;47(3):590-601. doi: 10.1023/a:1017976305128.
Repair of superficial damage to gastrointestinal mucosa occurs by a process called restitution. Goblet cells reside throughout the length of the intestine and are responsible for the production of mucus. However, a kinetic analysis of goblet cell dynamics of small intestine in restitution has hitherto not been reported. The aim of the present study was to investigate the role of goblet cells in the process of restitution of rat small intestine subjected to ischemia and ischemia-reperfusion injury, and therefore intestinal epithelium from rats subjected to both ischemia and ischemia-reperfusion was studied. Detachment of enterocytes was observed after 5-min of reperfusion. After 20-30 minutes of reperfusion, the denuded villous tips were covered with goblet cells. Within 75 min of reperfusion the epithelium restitution was complete. On the other hand, restitution was not observed in ischemia group. These data suggest that goblet cells may play an important role in restitution after ischemia-reperfusion injury.
胃肠道黏膜浅表损伤的修复通过一个称为再生的过程发生。杯状细胞存在于整个肠道长度中，负责产生黏液。然而，迄今为止尚未有关于再生过程中小肠杯状细胞动力学的动力学分析报道。本研究的目的是探讨杯状细胞在遭受缺血和缺血再灌注损伤的大鼠小肠再生过程中的作用，因此对遭受缺血和缺血再灌注的大鼠肠上皮进行了研究。再灌注5分钟后观察到肠上皮细胞脱离。再灌注20 - 30分钟后，裸露的绒毛尖端被杯状细胞覆盖。再灌注75分钟内上皮再生完成。另一方面，缺血组未观察到再生。这些数据表明杯状细胞可能在缺血再灌注损伤后的再生中起重要作用。
