Ou Xi-long, Sun Wei-hao, Xu Ming, Cao Da-zhong, Yu Qian, Yu Ting, Sun Yun-liang, Jiang Jie
Department of Gastroenterology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2005 Nov;8(6):524-7.
To investigate the expression of GST-pi and Topo II-alpha, and their relationships with clinicopathological parameters in colorectal carcinoma.
The expression of GST-pi and Topo II-alpha were detected by avidin-biotin-peroxide complex (ABC) method in tumor specimens, matched paratumor tissues from 60 cases with colorectal carcinoma and normal colonic tissues from 15 cases.
The expression rates of GST-pi and Topo II-alpha were 90.0% and 86.7% respectively in tumor tissues, significantly higher than those in matched paratumor tissues and normal tissues (P< 0.01). The expressions of GST-pi and Topo II-alpha were associated with cellular differentiation, Dukes stage and lymph node metastasis (all P< 0.01), but not with tumor size and histological type (all P > 0.05). The expression level of GST-pi was significantly higher in poorly differentiated tumors than that in well differentiated tumors. The expression level of Topo II-alpha in well-differentiated tumors were stronger than that in poorly differentiated tumors.
The detection of GST-pi and Topo II-alpha expressions may be helpful to judge the malignant behavior, metastasis and prognosis in human colorectal carcinoma.
探讨谷胱甘肽S转移酶π（GST-pi）和拓扑异构酶IIα（Topo II-α）在结直肠癌中的表达及其与临床病理参数的关系。
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法检测60例结直肠癌患者肿瘤标本、配对癌旁组织及15例正常结肠组织中GST-pi和Topo II-α的表达。
肿瘤组织中GST-pi和Topo II-α的表达率分别为90.0%和86.7%，显著高于配对癌旁组织和正常组织（P<0.01）。GST-pi和Topo II-α的表达与细胞分化、Dukes分期及淋巴结转移均相关（均P<0.01），但与肿瘤大小及组织学类型无关（均P>0.05）。低分化肿瘤中GST-pi的表达水平显著高于高分化肿瘤。高分化肿瘤中Topo II-α的表达水平强于低分化肿瘤。
检测GST-pi和Topo II-α的表达可能有助于判断人类结直肠癌的恶性行为、转移及预后。
