Chen Wan-yuan, Mao Wei-min, Zhao Li, Chen Guo-ping, Shu Yue, Shen Yu-fei, Zhu Xin-hai, Xia Yu
Department of Surgery, Zhejiang hospital, Hangzhou 310013, China.
Zhonghua Zhong Liu Za Zhi. 2005 Dec;27(12):738-40.
To study the expression and clinical significance of P-gp, GST-pi and Topo II alpha in gastric and colorectal cancers.
The expression of P-gp, GST-pi and Topo II alpha in 83 cases with gastric or colorectal cancer were examined by immunohistochemistry S-P.
The positive expression rates of P-gp, GST-pi, Topo II alpha in normal tissue and gastric and colorectal cancers were 69.9%, 65.1%, 50.6% and 83.1%, 85.5%, 45.8%, respectively. The positive rates of P-gp and GST-pi in gastric and colorectal cancer were significantly higher than those in normal gastric and colorectal tissue (P < 0.05). The expression of Topo II alpha in poorly differentiated cancers was significantly higher than that in well-and moderately differentiated cancers. There was no correlation between other items and clinicopathological parameters (P > 0.05).
P-gp, GST-pi and Topo II alpha play important role in multidrug resistance. Their mechanisms of drug resistance were different. The detection of expression of P-gp, GST-pi and Topo II alpha has an important guiding significance in chemotherapy for gastric and colorectal cancers.
研究P-糖蛋白（P-gp）、谷胱甘肽S转移酶π（GST-pi）和拓扑异构酶Ⅱα（TopoⅡα）在胃癌和结直肠癌中的表达及临床意义。
采用免疫组织化学S-P法检测83例胃癌或结直肠癌患者中P-gp、GST-pi和TopoⅡα的表达。
P-gp、GST-pi、TopoⅡα在正常组织及胃癌和结直肠癌中的阳性表达率分别为69.9%、65.1%、50.6%和83.1%、85.5%、45.8%。胃癌和结直肠癌中P-gp和GST-pi的阳性率显著高于正常胃和结直肠组织（P<0.05）。TopoⅡα在低分化癌中的表达显著高于高分化和中分化癌。其他各项与临床病理参数之间无相关性（P>0.05）。
P-gp、GST-pi和TopoⅡα在多药耐药中起重要作用。它们的耐药机制不同。检测P-gp、GST-pi和TopoⅡα的表达对胃癌和结直肠癌的化疗具有重要指导意义。
