Wilke A, Bartsch I, Kratz M, Jones D, Endres S
Klinik für Orthopädie und Rheumatologie der Philipps-Universität Marburg.
Biomed Tech (Berl). 2005 Oct;50(10):330-6. doi: 10.1515/BMT.2005.046.
There is considerable evidence that orthopaedic wear debris plays a crucial role in the pathology of aseptic loosening of joint prostheses. The purpose of the present study was to evaluate the influence of ultra-high-molecular-weight polyethylene (UHMW-PE) on the cytokine response in a modified in vitro model. UHMW-PE particles (psi < 7.5 microm) were suspended in soluble collagen type I and subsequently solidified in different concentrations (105,106 and 107 particles per well) on the bottom of the wells. Human bone marrow cells in a concentration of 3 x 106 cells per well were seeded on the collagen-particle substrata and maintained for up to 12 days. The cytokine response (IL-1_, IL-6 and TNF-_) of the cells to the particles were examined by ELISA compared to cells on control collagen surfaces without any particles. Assays for viability using LDH activity were done immediately. Light and scanning microscopic evaluation revealed that the UHMWPE particles, which have built large conglomerates (psi7.5_m), were mainly surrounded by the cells and less phagocytosed. The results of the cytokine release revealed significant differences in interleukin (IL)6, tumor necrosis factor (TNF)- _ and IL-1beta. The cell viability was not affected by the UHMW-PE particles. The results demonstrate that the particle induced cytokine response by UHMW-PE is mainly by the release of Interleukin 6 and TNF- _. Moreover the results confirm that the present method is useful to evaluate the in vitro effects of UHMW-PE wear particles with direct particle cell contact.
有大量证据表明，骨科磨损碎屑在关节假体无菌性松动的病理过程中起关键作用。本研究的目的是在改良的体外模型中评估超高分子量聚乙烯（UHMW-PE）对细胞因子反应的影响。将UHMW-PE颗粒（粒径<7.5微米）悬浮于I型可溶性胶原蛋白中，随后以不同浓度（每孔105、106和107个颗粒）在孔底部固化。将每孔浓度为3×106个细胞的人骨髓细胞接种于胶原蛋白颗粒基质上，并培养长达12天。与无颗粒的对照胶原蛋白表面上的细胞相比，通过酶联免疫吸附测定（ELISA）检测细胞对颗粒的细胞因子反应（IL-1β、IL-6和TNF-α）。立即使用乳酸脱氢酶（LDH）活性进行活力测定。光学显微镜和扫描显微镜评估显示，形成大团聚体（粒径>7.5微米）的UHMWPE颗粒主要被细胞包围，吞噬较少。细胞因子释放结果显示白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α和IL-1β有显著差异。细胞活力不受UHMW-PE颗粒影响。结果表明，UHMW-PE颗粒诱导的细胞因子反应主要是通过白细胞介素6和TNF-α的释放。此外，结果证实本方法可用于评估UHMW-PE磨损颗粒与细胞直接接触的体外效应。
