Wilke A, Endres S, Griss P, Herz U
Klinik für Orthopädie der Philipps-Universität Marburg, Germany.
Z Orthop Ihre Grenzgeb. 2002 Jan-Feb;140(1):83-9. doi: 10.1055/s-2002-22096.
The purpose of this study was to prove the effect of wear particles, especially Tivanium, in the mechanism of the aseptic loosening of total joint prostheses.
Therefore, human bone marrow cell cultures were incubated with titanium-aluminium-vanadium particles of different concentrations which were added after the seventh day of culture (10(9), 10(8), 10(7), 10(6) particles per ml medium). From this time starts the real culture period (2 weeks). During these two weeks the medium was changed and the supernatants were sampled. Using an ELISA the cytokine levels of interleukin-6, interleukin-1beta, TNF-alpha and LDH were measured approximately every second day (1, 3, 6, 8, 10, 14). As a marker for toxicity the activity of LDH was determined.
Incubation of a human bone marrow cell culture with titanium-aluminium-vanadium particles led to a maximum release of interleukin-6, interleukin-1beta, and TNF-alpha at high particle concentration (10(9) particles per ml medium). An increase of interleukin-1beta was only detectable at particle concentrations of 10(9) per ml medium. Exposure of the human bone marrow cell culture to titanium-aluminium-vanadium particles was toxic for high particle concentrations (10(9) particles per ml medium), as reflected by release of the intracellular enzyme LDH.
This study shows the ability of tivanium wear particles in a human bone marrow cell culture to induce a signfically higher release of proinflammatory and osteolytic mediators which are responsible for the aseptic loosening of prosthesis and the problem of revisions. In comparison to other cell studies, our results were explained by the human bone marrow cell culture. The human bone marrow is the real effector tissue source "in situ" because the prosthesis is localised intramedullarly.
本研究的目的是证明磨损颗粒,尤其是钛,在全关节假体无菌性松动机制中的作用。
因此,在培养的第7天之后添加不同浓度的钛铝钒颗粒(每毫升培养基中含10⁹、10⁸、10⁷、10⁶个颗粒),对人骨髓细胞培养物进行孵育。从此时开始进入实际培养期(2周)。在这两周内更换培养基并采集上清液。使用酶联免疫吸附测定法(ELISA)大约每隔一天(第1、3、6、8、10、14天)测量白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α和乳酸脱氢酶(LDH)的细胞因子水平。作为毒性标志物,测定LDH的活性。
用人骨髓细胞培养物与钛铝钒颗粒孵育,在高颗粒浓度(每毫升培养基中含10⁹个颗粒)时导致白细胞介素-6、白细胞介素-1β和肿瘤坏死因子-α的最大释放量。仅在每毫升培养基中颗粒浓度为10⁹时可检测到白细胞介素-1β的增加。高颗粒浓度(每毫升培养基中含10⁹个颗粒)时,人骨髓细胞培养物暴露于钛铝钒颗粒具有毒性,这通过细胞内酶LDH的释放得以体现。
本研究表明,在人骨髓细胞培养中,钛磨损颗粒能够诱导促炎和溶骨介质的显著更高释放,这些介质是假体无菌性松动和翻修问题的原因。与其他细胞研究相比,我们的结果用人骨髓细胞培养进行了解释。人骨髓是“原位”真正的效应组织来源,因为假体位于髓腔内。