Spichiger A C, Allenspach K, Ontsouka E, Gaschen F, Morel C, Blum J W, Sauter S N
Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, University of Bern, Bremgartenstrasse 109A, CH-3012 Bern, Switzerland.
J Vet Med A Physiol Pathol Clin Med. 2005 Dec;52(10):491-7. doi: 10.1111/j.1439-0442.2005.00770.x.
Repair processes of the inflamed intestine are very important for dissolution of chronic enteropathies (CE). Therefore, we examined the mRNA abundance of growth hormone receptor (GHR), insulin-like growth factors (IGF)-1 and -2 in duodenal and colonic biopsies of dogs with CE such as food-responsive diarrhoea (FRD) and inflammatory bowel disease (IBD) before and after treatment as compared with each other and healthy dogs. A clinical score (Canine IBD Activity Index = CIBDAI) was applied to judge the severity of CE. Biopsies of duodenum and colon from client-owned dogs with CE were sampled before (FRD(bef), n = 5; IBD(bef), n = 5) and after treatment (FRD(aft), n = 5; IBD(aft), n = 5). Intestinal control samples were available from a homogenous control population (n = 15; C). Intestinal samples were homogenized, total RNA was extracted, reverse transcribed and analysed by real-time polymerase chain reaction to measure mRNA levels of GHR, IGF-1 and IGF-2. Results were normalized with glyceraldehyde phosphate dehydrogenase as housekeeping gene. The CIBDAI decreased during the treatment period in FRD and IBD (P < 0.01). In duodenum, GHR mRNA levels were higher in all groups than in C (P < 0.001). Duodenal IGF-1 mRNA levels in FRD(aft) and IBD(aft) tended to be higher than in C (P < 0.1). The IGF-2 mRNA abundance in FRD(aft) was higher than in C (P < 0.05) in duodenum. In colon, mRNA levels of IGF-1 in IBD(aft) were higher than in FRD(aft) (P < 0.05) and levels differed between IBD(aft) and C (P < 0.05). In conclusion, mRNA levels of GHR, IGF-1 and IGF-2 in the gastrointestinal tract were increased during CE when compared with gastrointestinally healthy dogs. The data suggest that GHR, IGF-1 and IGF-2 are involved in gastrointestinal repair processes.
炎症肠道的修复过程对于慢性肠病（CE）的缓解非常重要。因此，我们检测了患有CE（如食物反应性腹泻（FRD）和炎症性肠病（IBD））的犬只在治疗前后十二指肠和结肠活检组织中生长激素受体（GHR）、胰岛素样生长因子（IGF）-1和-2的mRNA丰度，并将其相互比较，同时与健康犬只进行比较。应用临床评分（犬IBD活动指数 = CIBDAI）来判断CE的严重程度。从患有CE的客户拥有的犬只中采集十二指肠和结肠活检组织，在治疗前（FRD（bef），n = 5；IBD（bef），n = 5）和治疗后（FRD（aft），n = 5；IBD（aft），n = 5）进行采样。肠道对照样本来自同质对照群体（n = 15；C）。将肠道样本匀浆，提取总RNA，进行逆转录，并通过实时聚合酶链反应进行分析，以测量GHR、IGF-1和IGF-2的mRNA水平。结果以甘油醛-3-磷酸脱氢酶作为看家基因进行标准化。在FRD和IBD的治疗期间，CIBDAI下降（P < 0.01）。在十二指肠中，所有组的GHR mRNA水平均高于C组（P < 0.001）。FRD（aft）和IBD（aft）的十二指肠IGF-1 mRNA水平倾向于高于C组（P < 0.1）。在十二指肠中，FRD（aft）的IGF-2 mRNA丰度高于C组（P < 0.05）。在结肠中，IBD（aft）的IGF-1 mRNA水平高于FRD（aft）（P < 0.05），且IBD（aft）与C组之间存在差异（P < 0.05）。总之，与胃肠道健康的犬只相比，CE期间胃肠道中GHR、IGF-1和IGF-2的mRNA水平升高。数据表明GHR、IGF-1和IGF-2参与了胃肠道修复过程。
