Mima Takehiko, Sekiya Hiroshi, Mizushima Tohru, Kuroda Teruo, Tsuchiya Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan.
Microbiol Immunol. 2005;49(11):999-1002. doi: 10.1111/j.1348-0421.2005.tb03696.x.
We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.
我们通过聚合酶链反应（PCR）方法从铜绿假单胞菌中克隆了两个推测的RND型多药外排泵操纵子。我们将一个操纵子中的基因命名为mexPQ（-opmE），另一个操纵子中的基因命名为mexMN。将mexPQ-opmE导入药物超敏细胞导致大环内酯类、氟喹诺酮类和其他一些药物的最低抑菌浓度（MIC）升高。将mexMN导入具有oprM的超敏细胞中，而不是导入不具有oprM的细胞中，导致氯霉素和甲砜霉素的MIC升高。因此，我们得出结论，MexPQ-OpmE和MexMN-OprM在铜绿假单胞菌中表达时是功能性多药外排泵。
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