Murata Takeshi, Gotoh Naomasa, Nishino Takeshi
Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, 607-8414, Kyoto, Japan.
FEMS Microbiol Lett. 2002 Nov 19;217(1):57-63. doi: 10.1111/j.1574-6968.2002.tb11456.x.
The third genes, opmE, opmD and opmB, of multidrug efflux operons deduced from the Pseudomonas aeruginosa PAO1 genome data were cloned by polymerase chain reaction. The opmB gene product showed functional cooperation with inner membrane-associated components, MexAB, MexCD and MexXY, of the previously characterized multidrug efflux systems responsible for resistance to antimicrobial agents and extrusion of ethidium. The opmE and opmD gene products did not show functional cooperation. Immunoblots using a specific rabbit antiserum demonstrated, through exponential to stationary phases, constant expression of opmB and growth phase-dependent expression of opmD.
通过聚合酶链反应克隆了从铜绿假单胞菌PAO1基因组数据推导的多药外排操纵子的第三个基因opmE、opmD和opmB。opmB基因产物与先前表征的负责抗微生物剂抗性和溴化乙锭外排的多药外排系统的内膜相关成分MexAB、MexCD和MexXY表现出功能协同作用。opmE和opmD基因产物未表现出功能协同作用。使用特异性兔抗血清的免疫印迹表明，在指数期到稳定期，opmB持续表达，opmD表达呈生长阶段依赖性。
