使用高效离子交换色谱结合化学氧化/荧光检测法分析蓝藻源石房蛤毒素。

Analysis of cyanobacterial-derived saxitoxins using high-performance ion exchange chromatography with chemical oxidation/fluorescence detection.

作者信息

Papageorgiou John, Nicholson Brenton C, Linke Thomas A, Kapralos Con

机构信息

Australian Water Quality Centre, South Australian Water Corporation, Salisbury, South Australia, 5108, Australia.

出版信息

Environ Toxicol. 2005 Dec;20(6):549-59. doi: 10.1002/tox.20144.

DOI:10.1002/tox.20144

PMID:16302175

Abstract

A single run HPLC method utilizing ion exchange as the separation mode with a novel mobile phase system coupled to chemical postcolumn oxidation and fluorescence detection has been developed and demonstrated to be applicable to the quantitative analysis of paralytic shellfish poisons (PSPs) produced by Australian cyanobacteria (Anabaena circinalis) and other cyanobacteria. Both the cyanobacterial matrix and natural water constituents did not significantly affect the performance of this method. The daily precision of this method was adequate for it to be considered as a routine analytical tool for direct PSP analysis (prePSP concentration is not required) of cyanobacterial extracts and water bodies containing PSPs (C1, C2, GTX2, GTX3, NEO, STX) in the low parts per billion concentration range (10-70 ppb).

摘要

已开发出一种单次运行的高效液相色谱法，该方法以离子交换为分离模式，采用新型流动相系统，并结合化学柱后氧化和荧光检测，经证明适用于对澳大利亚蓝藻（卷曲鱼腥藻）及其他蓝藻产生的麻痹性贝类毒素（PSP）进行定量分析。蓝藻基质和天然水体成分均未对该方法的性能产生显著影响。该方法的日常精密度足以使其被视为一种常规分析工具，用于直接对蓝藻提取物和含有低十亿分浓度范围（10 - 70 ppb）的PSP（C1、C2、GTX2、GTX3、NEO、STX）的水体进行PSP分析（无需预PSP浓度）。