Botti Paolo, Tchertchian Sylvie
Department of Structural Biology and Bioinformatics, University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland.
Protein Pept Lett. 2005 Nov;12(8):729-35. doi: 10.2174/0929866054864300.
An overview of the applications of Nalpha-(1-phenyl-2-mercaptoethyl) auxiliary is presented. We describe the on resin preparation (Calpha-carboxy and thioester) of Nalpha-auxiliary derivatives of glycine and the synthesis and incorporation of preformed Nalpha-auxiliary derivatives of glycine and alanine with the protection schemes, including the thiazolidine strategy for SPPS. Such approaches allowed the synthesis of the protein cytochrome b562 as well as native circular peptides after successful removal of the auxiliary.
本文介绍了Nα-(1-苯基-2-巯基乙基)辅助剂的应用概况。我们描述了甘氨酸Nα-辅助衍生物在树脂上的制备（α-羧基和硫酯），以及甘氨酸和丙氨酸预制Nα-辅助衍生物的合成与引入及其保护方案，包括固相肽合成的噻唑烷策略。这些方法使得在成功去除辅助剂后能够合成蛋白质细胞色素b562以及天然环肽。
