Expressed protein ligation to obtain selectively modified aldo/keto reductases.

作者信息

Richter Michael P O, Beck-Sickinger Annette G

机构信息

Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.

出版信息

Protein Pept Lett. 2005 Nov;12(8):777-81. doi: 10.2174/0929866054864210.

DOI:10.2174/0929866054864210

PMID:16305548

Abstract

Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

摘要