Mini Roberta, Annibale Bruno, Lahner Edith, Bernardini Giulia, Figura Natale, Santucci Annalisa
Dipartimento di Biologia Molecolare, Policlinico Le Scotte, Università degli Studi di Siena, Siena, Italy.
Clin Chem. 2006 Feb;52(2):220-6. doi: 10.1373/clinchem.2005.054627. Epub 2005 Nov 23.
Atrophic body gastritis is considered the first important step in the histogenesis of gastric carcinoma, a multistep process starting from chronic gastritis and progressing through chronic atrophic gastritis, intestinal metaplasia, and dysplasia. Helicobacter pylori is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because of the progressive disappearance of the bacterium. Our study aimed to detect past H. pylori infection in patients with atrophic body gastritis.
We used Western blot analyses of whole bacterial protein lysate of 2 different strains to probe sera from 143 patients. All sera were analyzed by ELISA (Bio-Rad), and results of gastric histology were available for all patients.
Among 111 patient sera previously classified as negative for H. pylori infection by ELISA, 106 (95.5%) were positive when assayed by immunoblotting.
Commercial diagnostic reagent sets may fail to detect H. pylori infection. Western blotting of whole bacterial protein extracts could provide the basis of a noninvasive serology tool able to assess previous infection with H. pylori in patients with atrophic body gastritis.
萎缩性胃体炎被认为是胃癌组织发生过程中重要的起始步骤，这是一个从慢性胃炎开始，经过慢性萎缩性胃炎、肠化生和发育异常的多步骤过程。幽门螺杆菌参与萎缩性胃体炎的诱发，但由于该细菌逐渐消失，幽门螺杆菌感染的记录较为困难。我们的研究旨在检测萎缩性胃体炎患者过去的幽门螺杆菌感染情况。
我们使用两种不同菌株的全菌蛋白裂解物进行蛋白质印迹分析，以检测143例患者的血清。所有血清均通过酶联免疫吸附测定（Bio-Rad）进行分析，并且所有患者均有胃组织学检查结果。
在之前酶联免疫吸附测定判定为幽门螺杆菌感染阴性的111例患者血清中，通过免疫印迹法检测时，有106例（95.5%）呈阳性。
商业诊断试剂盒可能无法检测出幽门螺杆菌感染。全菌蛋白提取物的蛋白质印迹法可为评估萎缩性胃体炎患者既往幽门螺杆菌感染情况的非侵入性血清学工具提供依据。
