Schiavone Maria Lucia, Barletta Roberta, Trezza Alfonso, Geminiani Michela, Millucci Lia, Figura Natale, Santucci Annalisa
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro, 53100 Siena, Italy.
SienabioACTIVE, Università di Siena, Via Aldo Moro, 53100 Siena, Italy.
Molecules. 2025 Jan 15;30(2):324. doi: 10.3390/molecules30020324.
Green chemistry principles are pivotal in driving sustainable and innovative solutions to global health challenges. This study explores a hydroalcoholic extract from (chestnut) burrs, an underutilized natural resource, as a potent source of antimicrobial compounds against (). The extract demonstrated significant bactericidal activity, synergizing effectively with clarithromycin and showing additive effects with metronidazole. Remarkably, combining the extract with clarithromycin and sub-inhibitory concentrations of pantoprazole reduced clarithromycin's Minimum Bactericidal Concentration (MBC) to just 1.56% of its original value. Mechanistic studies suggest that the extract's polyphenolic compounds compromise bacterial membrane integrity, enhancing antibiotic uptake, while pantoprazole disrupts bacterial ATPase activity. This research highlights the critical role of natural product extraction within the framework of green chemistry, offering a sustainable and environmentally friendly alternative to synthetic antimicrobials. By harnessing bioactive compounds from plant sources, this approach addresses the pressing issue of antibiotic resistance while promoting the responsible use of natural resources. The findings underscore the transformative potential of green chemistry in developing effective, eco-conscious antimicrobial therapies that align with global sustainability goals.
绿色化学原理对于推动解决全球健康挑战的可持续和创新解决方案至关重要。本研究探索了一种来自板栗刺（一种未充分利用的自然资源）的水醇提取物，作为对抗幽门螺杆菌的有效抗菌化合物来源。该提取物表现出显著的杀菌活性，与克拉霉素有效协同，并与甲硝唑显示出相加作用。值得注意的是，将该提取物与克拉霉素以及亚抑菌浓度的泮托拉唑联合使用，可将克拉霉素的最低杀菌浓度（MBC）降至其原始值的仅1.56%。机理研究表明，该提取物的多酚类化合物破坏细菌膜完整性，增强抗生素摄取，而泮托拉唑则破坏细菌ATP酶活性。这项研究突出了绿色化学框架内天然产物提取的关键作用，为合成抗菌剂提供了一种可持续且环保的替代方案。通过利用植物来源的生物活性化合物，这种方法解决了抗生素耐药性这一紧迫问题，同时促进了自然资源的合理利用。研究结果强调了绿色化学在开发符合全球可持续发展目标的有效、具有生态意识的抗菌疗法方面的变革潜力。
