Kresse J I, Stewart W C, Carbrey E A, Snyder M L
Am J Vet Res. 1975 Feb;36(2):141-4.
A 2-step technique for the isolation of hog cholera (HC) virus consisting of an initial culture on buffy coat (BC) cultures and subinoculation to a pig kidney cell line (PK-15) was described. By this technique, HC virus was confirmed in specimens from 65 herds in which the conventional cell culture isolation technique failed. The herds were located in 20 states and Puerto Rico. Specimens from 29 of the 65 herds were inoculated into specific-pathogen-free (SPF) pigs. Hog cholera virus was recovered from 27 of the test pigs. The 2 pigs from which virus was not recovered had signs of acute infection and, on necropsy, had gross lesions of HC infection.
描述了一种用于分离猪霍乱（HC）病毒的两步技术，该技术包括首先在血沉棕黄层（BC）培养物上培养，然后传代接种到猪肾细胞系（PK - 15）。通过这种技术，在65个畜群的样本中证实了HC病毒，而在这些畜群中，传统的细胞培养分离技术失败了。这些畜群分布在20个州和波多黎各。65个畜群中29个畜群的样本接种到无特定病原体（SPF）猪体内。从27只试验猪中分离出了猪霍乱病毒。未分离出病毒的2只猪有急性感染症状，尸检时有HC感染的肉眼病变。
