猪自然感染牛病毒性腹泻病毒及其与猪瘟的鉴别诊断。

Natural infection of pigs with bovine viral diarrhea virus and its differential diagnosis from hog cholera.

作者信息

Carbrey E A, Stewart W C, Kresse J I, Snyder M L

出版信息

J Am Vet Med Assoc. 1976 Dec 1;169(11):1217-9.

Abstract

Natural infection of pigs with bovine viral diarrhea virus (BVDV) through contact with infected cattle has caused problems in diagnosing hog cholera (HC). Low cross-reacting serum antibody titers against HC caused by BVDV infection were found in clinically normal pigs as well as those suspected of having HC. Bovine viral diarrhea virus was isolated from specimen tissues and initially identified as HC virus (HCV), using the fluorescent antibody cell culture technique. Additional cell cultures, as well as pig and calf trials, were necessary to identify it as BVDV. The isolate caused clinical signs of illness in the calves, whereas the pigs remained healthy. Bovine viral diarrhea virus may be detected in tissue sections or isolated in cell cultures and confirmed as HCV, using the HC fluorescent antibody conjugate. Laboratories performing the neutralization test for HC should use discretion when interpreting HC titers unless BVD titers are determined on the same serums.

摘要

猪通过与感染牛接触而自然感染牛病毒性腹泻病毒（BVDV），给猪瘟（HC）的诊断带来了问题。在临床正常的猪以及疑似患有猪瘟的猪中，均发现了由BVDV感染引起的针对猪瘟的低交叉反应血清抗体滴度。从标本组织中分离出牛病毒性腹泻病毒，并使用荧光抗体细胞培养技术初步鉴定为猪瘟病毒（HCV）。需要进行额外的细胞培养以及猪和小牛试验，才能将其鉴定为BVDV。该分离株在小牛身上引起了疾病的临床症状，而猪则保持健康。使用猪瘟荧光抗体结合物，可在组织切片中检测到牛病毒性腹泻病毒，或在细胞培养物中分离出来并确认为猪瘟病毒。进行猪瘟中和试验的实验室在解释猪瘟滴度时应谨慎行事，除非对同一份血清测定了牛病毒性腹泻病毒滴度。