Ishida-Fujii Keiko, Goto Shingo, Uemura Ryu, Yamada Keita, Sato Michikatsu, Yoshida Naohiro
R and D Center, Alcohol Enterprise Head Office, New Energy and Industrial Technology Development Organization, Chiba, Japan.
Biosci Biotechnol Biochem. 2005 Nov;69(11):2193-9. doi: 10.1271/bbb.69.2193.
The isotope ratios of carbon, hydrogen, and oxygen of rectified alcohols were determined to distinguish their botanical and geographical origins by continuous flow-isotope ratio mass spectrometry (CF-IRMS). The (13)C/(12)C and (18)O/(16)O ratios of 27 fermented alcohols with known origins showed clusters derived from each botanical origin, viz. corn, sugarcane, wheat, and tapioca. C3 and C4 plants were easily distinguishable by the (13)C/(12)C ratio. Sugarcane and corn are both C4 plants, and they showed small differences in isotope ratios. The combination plots of the D/H and (18)O/(16)O ratios enabled us to designate the geographical origins of alcohol derived from the same kind of crop, such as Chinese or American corn. The chemically synthetic and fermented alcohols were clearly distinguished by D/H and (18)O/(16)O ratios. Isotope ratios were useful for origin identification of alcohol. We plan to construct a database of alcohol isotope ratios to determine the origins of raw materials in alcohol.
采用连续流同位素比率质谱法（CF-IRMS）测定精馏酒精中碳、氢和氧的同位素比率，以区分其植物来源和地理来源。对27种已知来源的发酵酒精的(13)C/(12)C和(18)O/(16)O比率进行分析，结果显示源自不同植物来源（即玉米、甘蔗、小麦和木薯）的酒精呈现出各自的聚类。通过(13)C/(12)C比率可以轻松区分C3和C4植物。甘蔗和玉米均为C4植物，它们的同位素比率存在细微差异。D/H和(18)O/(16)O比率的组合图使我们能够确定源自同一种作物（如中国玉米或美国玉米）的酒精的地理来源。化学合成酒精和发酵酒精可通过D/H和(18)O/(16)O比率进行明确区分。同位素比率有助于酒精的来源鉴定。我们计划构建一个酒精同位素比率数据库，以确定酒精中原材料的来源。
