Novák P, Zahradník I
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, 833 34 Bratislava 37, Slovakia.
Gen Physiol Biophys. 2005 Sep;24(3):337-48.
The conventional patch-clamp technique requires well-trained experimenter. Few commercial automated patch-clamp systems, designed for drug development, are better suited for large-scale research then for standard electrophysiological experiments. Here we describe a state machine for automated recognition of recording states of the patch-clamp experiment. The principle of the state machine is based on evaluation of the charge carried by membrane current during specific time segments in responses to square wave voltage stimulation. The state machine may serve for generating various sound alerts, signals for automated control of other devices, assistance in micromanipulation, internal pipette pressure control, and holding potential adjustments. Algorithm of the state machine, designed to cover wide variety of cell types, was successfully tested on rat ventricular myocytes.
传统的膜片钳技术需要训练有素的实验人员。很少有专为药物开发设计的商用自动膜片钳系统更适合大规模研究，而非标准电生理实验。在此，我们描述一种用于自动识别膜片钳实验记录状态的状态机。该状态机的原理基于在对方波电压刺激的响应中，对特定时间段内膜电流所携带电荷的评估。该状态机可用于生成各种声音警报、用于其他设备自动控制的信号、辅助显微操作、内部移液器压力控制以及保持电位调整。旨在涵盖多种细胞类型的状态机算法已在大鼠心室肌细胞上成功测试。
