Aquaporin A/S, Diplomvej 377, DK-2800 Kgs. Lyngby, Denmark.
Bioinspir Biomim. 2009 Dec;4(4):044001. doi: 10.1088/1748-3182/4/4/044001. Epub 2009 Nov 9.
Recent advances in biomimetic membrane systems have resulted in an increase in membrane lifetimes from hours to days and months. Long-lived membrane systems demand the development of both new automated monitoring equipment capable of measuring electrophysiological membrane characteristics and new data processing software to analyze and organize the large amounts of data generated. In this work, we developed an automated instrumental voltage clamp solution based on a custom-designed software controller application (the WaveManager), which enables automated on-line voltage clamp data acquisition applicable to long-time series experiments. We designed another software program for off-line data processing. The automation of the on-line voltage clamp data acquisition and off-line processing was furthermore integrated with a searchable database (DiscoverySheet) for efficient data management. The combined solution provides a cost efficient and fast way to acquire, process and administrate large amounts of voltage clamp data that may be too laborious and time consuming to handle manually.
仿生膜系统的最新进展使得膜的寿命从小时延长到几天甚至几个月。长寿命膜系统需要开发新的自动化监测设备，以测量电生理膜特性，以及新的数据处理软件来分析和组织产生的大量数据。在这项工作中，我们开发了一种基于定制软件控制器应用程序（WaveManager）的自动化仪器电压钳制解决方案，该解决方案能够实现适用于长时间序列实验的自动化在线电压钳制数据采集。我们还设计了另一个用于离线数据处理的软件程序。在线电压钳数据采集和离线处理的自动化还与一个可搜索数据库（DiscoverySheet）集成，用于高效的数据管理。该组合解决方案提供了一种经济高效且快速的方法来获取、处理和管理大量的电压钳制数据，这些数据如果手动处理可能过于繁琐和耗时。
