Paulsen Jan Erik, Namork Ellen, Alexander Jan
Department of Food Toxicology, Norwegian Institute of Public Health, 0403 Oslo, Norway.
Anticancer Res. 2005 Nov-Dec;25(6B):3883-8.
The surface morphology of late colonic lesions in F344 rats treated with 1,2-dimethylhydrazine was studied by scanning electron microscopy. At week 31 after carcinogen treatment, the surface epithelial characteristics of different types of lesions observed in the colonic mucosa were compared, namely classic elevated aberrant crypt foci (ACF), flat lesion and gross tumour. Classic elevated ACF were easily observed as structures with enlarged crypts elevated from the background mucosa. When the various ACF were compared, or when the ACF were compared with the background mucosa, no ultrastructural differences, or differences in the density of goblet cells were found. The flat lesion showed an epithelium without goblet cells and crypts with small openings harbouring a large number of loose, undefined, dysplastic epithelial cells. These changes appeared to be linked to the malignant development since they were also characteristic of the examined tumour.
通过扫描电子显微镜研究了用1,2 - 二甲基肼处理的F344大鼠晚期结肠病变的表面形态。在致癌物处理后的第31周，比较了在结肠黏膜中观察到的不同类型病变的表面上皮特征，即典型的隆起性异常隐窝灶（ACF）、扁平病变和大体肿瘤。典型的隆起性ACF很容易被观察到是从背景黏膜中隆起的隐窝增大的结构。当比较各种ACF时，或者当将ACF与背景黏膜进行比较时，未发现超微结构差异或杯状细胞密度差异。扁平病变显示上皮无杯状细胞，隐窝开口小，含有大量松散、未定型的发育异常上皮细胞。这些变化似乎与恶性发展有关，因为它们也是所检查肿瘤的特征。
