McLellan E A, Medline A, Bird R P
Histopathology Unit, Imperial Cancer Research Foundation, London, United Kingdom.
Cancer Res. 1991 Oct 1;51(19):5270-4.
The main objective of the present study was to sequentially analyze growth and morphological characteristics of aberrant crypt foci (ACF) in the rat colon. Sprague-Dawley rats were given a single injection of a carcinogenic dose of 1,2-dimethylhydrazine-HCl and at varying time points ranging from 2 to 57 weeks, groups of 5 rats were terminated. The number and crypt multiplicity of ACF were determined in the distal 8 cm of the colon. In addition, ACF were processed for histology and then graded for the presence of nuclear atypia using a score of 0-4. The findings of the present study demonstrated that ACF exhibit the characteristics expected for precursor lesions. ACF were present at all time intervals in large numbers in the colons of rats treated with 1,2-dimethylhydrazine-HCl and were present when adenocarcinomas were observed. The number of ACF with 4 or more crypts and those exhibiting a higher grade (grade 4) of nuclear atypia increased significantly at or beyond 19 weeks. These features of ACF, particularly the presence of nuclear atypia indicative of dysplasia, provide strong support for the hypothesis that ACF are precursor lesions of chemically induced colon cancer.
本研究的主要目的是对大鼠结肠中异常隐窝病灶（ACF）的生长和形态特征进行序贯分析。给Sprague-Dawley大鼠单次注射致癌剂量的盐酸1,2-二甲基肼，在2至57周的不同时间点，每组5只大鼠被处死。测定结肠远端8厘米处ACF的数量和隐窝多倍性。此外，对ACF进行组织学处理，然后使用0至4分的评分对核异型性的存在进行分级。本研究结果表明，ACF表现出前体病变预期的特征。在接受盐酸1,2-二甲基肼治疗的大鼠结肠中，ACF在所有时间间隔都大量存在，并且在观察到腺癌时也存在。具有4个或更多隐窝以及表现出更高核异型性等级（4级）的ACF数量在19周及以后显著增加。ACF的这些特征，特别是表明发育异常的核异型性的存在，为ACF是化学诱导结肠癌的前体病变这一假说提供了有力支持。
