Lin Yi, Chen Yijing, Zeng Yaoying, Wang Tong, Zeng Shan
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou City 510632, China.
J Reprod Immunol. 2005 Dec;68(1-2):39-51. doi: 10.1016/j.jri.2005.05.002. Epub 2005 Nov 23.
The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID x NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID x NOD/SCID mice and compared with non-immunodeficiency control BALB/c x BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID x NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID x NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy.
本研究的目的是概述非肥胖糖尿病（NOD）/LtSz - scid/scid（简称为NOD/SCID[严重联合免疫缺陷]）小鼠的生育特征，并评估自然杀伤（NK）细胞亚群对同基因NOD/SCID×NOD/SCID交配组合妊娠结局的影响。首先，采用流式细胞术进行淋巴细胞表型分析，以确认NOD/SCID小鼠存在多种免疫缺陷。对NOD/SCID×NOD/SCID小鼠的生育特征进行评估，并与非免疫缺陷对照BALB/c×BALB/c小鼠进行比较。尽管在NOD/SCID小鼠中NK细胞缺陷明显，但在这些小鼠中仍可观察到一定水平的残余NK活性。分别用聚肌苷酸 - 聚胞苷酸（polyIC）刺激或用抗唾液酸GM1（ASGM1）抗血清抑制残余NK细胞活性。给药后评估这些因素对妊娠结局的影响。在NOD/SCID×NOD/SCID小鼠中可观察到大致正常的生育能力。然而，与对照NOD/SCID小鼠相比，polyIC处理的NOD/SCID×NOD/SCID小鼠的窝仔数略大。相反，注射ASGM1后胚胎吸收增加，随后与较小的窝仔数相关。这表明NOD/SCID小鼠中的残余NK细胞活性可能有利于孕期母胎耐受。
