Djordjevic Jelena, Cvijic Gordana, Petrovic Natasa, Davidovic Vukosava
Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Studentski Trg 16, PO BOX 52, 11000 Belgrade, Serbia and Montenegro.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Dec;142(4):433-8. doi: 10.1016/j.cbpa.2005.09.014. Epub 2005 Nov 23.
Our previous results have shown that metabolic and thermal stressors influence interscapular brown adipose tissue (IBAT) metabolic activity by increasing oxygen consumption and, consequently, altering the toxic reactive oxygen species (ROS) production and the antioxidative system activity. Since there is not enough evidence about the effect of psychosocial stressors on these processes, we studied the effect of acute crowding stress on the IBAT and hypothalamic monoamine oxidase (MAO) activity as well as IBAT antioxidative enzymes, manganese (MnSOD), copper-zinc superoxide dismutase (CuZnSOD) and catalase (CAT), as the relevant indicators of IBAT metabolic alternations under the stress exposure and the returning of animals to control conditions. The results indicated that acute crowding stress did not change the hypothalamic and IBAT MAO activities, the generation of ROS and, consequently, the IBAT CuZnSOD and CAT activities. However, all three antioxidative enzymes were affected only after the recovery period. It seems that peripheral overheating of rats during acute crowding changes the stress nature, by becoming more thermal than psychosocial and by suppression the hypothalamic efferent pathways involved in the IBAT thermogenesis regulation. However, it seems that returning of the animals to the control conditions after the stress termination causes the reactivation of IBAT thermogenesis with tendency to normalise the body temperature.
我们之前的研究结果表明，代谢应激源和热应激源通过增加耗氧量来影响肩胛间棕色脂肪组织（IBAT）的代谢活性，进而改变有毒活性氧（ROS）的产生以及抗氧化系统的活性。由于关于心理社会应激源对这些过程影响的证据不足，我们研究了急性拥挤应激对IBAT和下丘脑单胺氧化酶（MAO）活性以及IBAT抗氧化酶、锰超氧化物歧化酶（MnSOD）、铜锌超氧化物歧化酶（CuZnSOD）和过氧化氢酶（CAT）的影响，这些酶是应激暴露及动物恢复到对照条件下IBAT代谢变化的相关指标。结果表明，急性拥挤应激并未改变下丘脑和IBAT的MAO活性、ROS的生成以及IBAT的CuZnSOD和CAT活性。然而，仅在恢复期后，这三种抗氧化酶均受到影响。在急性拥挤期间，大鼠的外周过热似乎改变了应激的性质，使其热应激成分超过心理社会应激成分，并抑制了参与IBAT产热调节的下丘脑传出通路。然而，应激终止后动物恢复到对照条件似乎会导致IBAT产热重新激活，并使体温趋于正常。
