寡糖的快速测序：试剂阵列分析方法。
Fast sequencing of oligosaccharides: the reagent-array analysis method.
作者信息
Edge C J, Rademacher T W, Wormald M R, Parekh R B, Butters T D, Wing D R, Dwek R A
机构信息
Department of Biochemistry, University of Oxford, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6338-42. doi: 10.1073/pnas.89.14.6338.
Abstract
A method of oligosaccharide analysis involving controlled fragmentation resulting from enzymatic digestion is presented. The principle involves generating a set of fragments from the original oligosaccharides, characterizing them in terms of their hydrodynamic volumes, determining their molar proportions, and identifying the oligosaccharides by comparison with a computer-generated data base. Experimentally, this technique involves incubation of aliquots of a sample with a set of defined mixtures of exoglycosidases followed by pooling of the products and a single analysis on the product pool. This method has several practical advantages over current techniques, including speed and the ability to use smaller amounts of starting material. The detection of the intensity-versus-hydrodynamic volume profile is limited only by the specific activity of the labeling method. The ability to perform the enzyme digestions is limited by the individual Km values of the enzymes.
摘要
本文介绍了一种涉及酶消化产生可控片段的寡糖分析方法。其原理包括从原始寡糖生成一组片段，根据它们的流体力学体积对其进行表征，确定它们的摩尔比例，并通过与计算机生成的数据库进行比较来鉴定寡糖。在实验中，该技术涉及将样品的等分试样与一组确定的外切糖苷酶混合物一起孵育，然后合并产物并对产物池进行单一分析。与当前技术相比，该方法具有几个实际优势，包括速度快以及能够使用较少量的起始材料。强度与流体力学体积曲线的检测仅受标记方法的比活性限制。进行酶消化的能力受酶的各个 Km 值限制。
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本文引用的文献
1
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Methods Enzymol. 1982;83:105-26. doi: 10.1016/0076-6879(82)83008-5.
2
Substrate specificity and other properties of alpha-L-fucosidase from human serum.人血清α-L-岩藻糖苷酶的底物特异性及其他特性
J Biol Chem. 1982 Jan 25;257(2):714-8.
3
On the specificity of the two forms of human serum alpha-L-fucosidase.
Int J Biochem. 1983;15(11):1385-7. doi: 10.1016/0020-711x(83)90031-9.
4
F.A.B.-mass spectrometry of carbohydrates.碳水化合物的F.A.B.质谱分析
Adv Carbohydr Chem Biochem. 1987;45:19-72. doi: 10.1016/s0065-2318(08)60136-5.
5
Glycobiology.糖生物学
Annu Rev Biochem. 1988;57:785-838. doi: 10.1146/annurev.bi.57.070188.004033.
6
Tissue-specific N-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat Thy-1.大鼠Thy-1的组织特异性N-糖基化、位点特异性寡糖模式及扁豆凝集素识别
EMBO J. 1987 May;6(5):1233-44. doi: 10.1002/j.1460-2075.1987.tb02359.x.
7
The isolation by ligand affinity chromatography of a novel form of alpha-L-fucosidase from almond.通过配体亲和色谱法从杏仁中分离出一种新型α-L-岩藻糖苷酶。
J Biol Chem. 1990 Sep 25;265(27):16472-7.
8
Purification to homogeneity of Charonia lampas alpha-fucosidase by using sequential ligand-affinity chromatography.通过连续配体亲和色谱法将皱纹岩螺α-岩藻糖苷酶纯化至同质。
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):189-95. doi: 10.1042/bj2790189.
9
Glycosylation of interleukin-6 purified from normal human blood mononuclear cells.从正常人血液单核细胞中纯化的白细胞介素-6的糖基化作用。
Eur J Biochem. 1992 Jan 15;203(1-2):135-41. doi: 10.1111/j.1432-1033.1992.tb19838.x.
10
The asparagine-linked sugar chains of subcomponent C1q of the first component of human complement.
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