Zhao Y, Kent S B, Chait B T
The Rockefeller University, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1629-33. doi: 10.1073/pnas.94.5.1629.
We have developed an efficient method for the derivatization of oligosaccharides, wherein the oligosaccharide is efficiently ligated to a basic aminooxyacetyl peptide by oxime formation. The resulting glycopeptide yields much higher sensitivity in matrix-assisted laser desorption/ionization mass spectrometry than does the underivatized oligosaccharide. Digestion of the glycopeptide by a exoglycosidase array and subsequent mass spectrometric assay of the digestion products provide a sensitive and rapid way to elucidate the structure of the oligosaccharide. In addition to oligosaccharide sequencing, the ligation reaction between an oligosaccharide and an aminooxyacetyl peptide also provides a potentially very convenient and efficient way for the synthesis of glycopeptides or glycoproteins.
我们已经开发出一种用于寡糖衍生化的高效方法，其中寡糖通过肟形成有效地连接到碱性氨氧基乙酰肽上。所得糖肽在基质辅助激光解吸/电离质谱中产生的灵敏度比未衍生化的寡糖高得多。通过外切糖苷酶阵列消化糖肽并随后对消化产物进行质谱分析，为阐明寡糖的结构提供了一种灵敏且快速的方法。除了寡糖测序外，寡糖与氨氧基乙酰肽之间的连接反应还为糖肽或糖蛋白的合成提供了一种潜在非常方便且高效的方法。
