Zhang Qunlin, Myint Aung, Cui Hua, Ge Xuewu, Liu Lijuan, Chou Guixin
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui.
Phytochem Anal. 2005 Nov-Dec;16(6):440-5. doi: 10.1002/pca.867.
A sensitive and selective flow injection chemiluminescence method for the determination of cardamonin over the range 1.0 x 10(-8) to 8.0 x 10(-6) g/mL is described. The method is based on the enhancement by cardamonin of the chemiluminescence of the reaction between cerium (IV) and rhodamine 6G in sulphuric acid medium. The optimised flow injection procedure yielded a detection limit for cardamonin of 8.8 x 10(-9) g/mL, whilst the relative standard deviations of intraday and inter-day precision were below 2.5%. The method has the advantages of high sensitivity and a wide linear range. It was successfully applied to the determination of cardamonin in Alpinia katsumadai Hayata. The mechanism of the chemiluminescence reaction is proposed.
描述了一种灵敏且选择性的流动注射化学发光法，用于测定小豆蔻明，其测定范围为1.0×10⁻⁸至8.0×10⁻⁶ g/mL。该方法基于在硫酸介质中小豆蔻明对铈（IV）与罗丹明6G反应化学发光的增强作用。优化后的流动注射程序得出小豆蔻明的检测限为8.8×10⁻⁹ g/mL，日内和日间精密度的相对标准偏差均低于2.5%。该方法具有灵敏度高和线性范围宽的优点。它已成功应用于草豆蔻中小豆蔻明的测定。并提出了化学发光反应的机理。
