一种基于Ru(phen)3(2+)-Ce(IV)体系的简便快速流动注射化学发光法测定那可丁

A simple and rapid flow-injection chemiluminescence method for the determination of noscapine with Ru(phen)3(2+)-Ce(IV) system.

作者信息

Rezaei Behzad, Mokhtari Ali, Khayamian Taghi

机构信息

Department of Chemistry, Isfahan University of Technology, 84156 Isfahan I.R. Iran.

出版信息

Ann Chim. 2007 Aug;97(8):605-14. doi: 10.1002/adic.200790046.

DOI:10.1002/adic.200790046

PMID:17899875

Abstract

A new flow injection chemiluminescence (CL) system was used for the determination of noscapine. This technique is based on the reduction effect of noscapine on the Ru(phen)3(3+), which is produced by reaction between Ru(phen)3(2+) and acidic Ce(IV) solutions, and this rapid reduction produces strong CL. Calibration plots were linear over the range of 3.0 x 10(-7) - 2.0 x 10(-6) mol L(-1) and 2.0 x 10(-6) - 2.0 x 10(-4) mol L(-1). The CL intensity was so high, that it is able to produce a detection limit of 6.6 x 10(-8) M noscapine (3sigma). The relative standard deviation of 2.0 x 10(-6) M noscapine was 1.0% (n=10). The proposed method was successfully applied for the flow injection determination of noscapine in cough and Tonin syrup samples. The results of real sample analyses show good recovery percentages (97.3-102.4%). The minimum sampling rate was 100 samples per hour.

摘要

一种新的流动注射化学发光（CL）系统用于那可丁的测定。该技术基于那可丁对Ru(phen)3(3+)的还原作用，Ru(phen)3(3+)由Ru(phen)3(2+)与酸性Ce(IV)溶液反应产生，这种快速还原产生强烈的化学发光。校准曲线在3.0×10(-7) - 2.0×10(-6) mol L(-1)和2.0×10(-6) - 2.0×10(-4) mol L(-1)范围内呈线性。化学发光强度很高，能够产生那可丁的检测限为6.6×10(-8) M（3σ）。2.0×10(-6) M那可丁的相对标准偏差为1.0%（n = 10）。所提出的方法成功应用于咳嗽糖浆和托宁糖浆样品中那可丁的流动注射测定。实际样品分析结果显示出良好的回收率（97.3 - 102.4%）。最小采样率为每小时100个样品。